Itoh K, Ozaki A, Yamamoto T, Mitsuoka T
Jikken Dobutsu. 1978 Jan;27(1):13-6. doi: 10.1538/expanim1978.27.1_13.
A lightweight stainless steel autoclavable pentagon isolator was designed for experiments using gnotobiotic mice. The chamber, 400 x 450 x 350 mm, has an entry port 200 mm in diameter at the back and a window at the ceiling. The globes and two filters were equipped at the front and each side, respectively. An inner stainless steel cap of the entry lock was sealed by a seamless silicone band. It was possible to ventilate this isolator by either free-flow or blower operation. After autoclaving the isolators 10 to 16 times for a year, none of them was repaired. Five mice can be kept in this isolator for about 1 month without supply of diet, water and wood shaving after the first setting.
设计了一种用于无菌小鼠实验的轻质不锈钢可高压灭菌五边形隔离器。该隔离器的腔室尺寸为400×450×350毫米,背面有一个直径200毫米的入口,天花板上有一扇窗户。前面和两侧分别装有玻璃罩和两个过滤器。入口锁的内部不锈钢盖由无缝硅胶带密封。该隔离器可以通过自由流动或鼓风机操作进行通风。一年中对这些隔离器进行10至16次高压灭菌后,没有一个需要维修。首次放置后,五只小鼠可以在这个隔离器中饲养约1个月,无需供应食物、水和木屑。