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用于小规模悉生生物学实验的可高压灭菌不锈钢隔离器(作者译)

[An autoclavable stainless steel isolator for small scale gnotobiotic experiments (author's transl)].

作者信息

Itoh K, Ozaki A, Yamamoto T, Mitsuoka T

出版信息

Jikken Dobutsu. 1978 Jan;27(1):13-6. doi: 10.1538/expanim1978.27.1_13.

DOI:10.1538/expanim1978.27.1_13
PMID:580242
Abstract

A lightweight stainless steel autoclavable pentagon isolator was designed for experiments using gnotobiotic mice. The chamber, 400 x 450 x 350 mm, has an entry port 200 mm in diameter at the back and a window at the ceiling. The globes and two filters were equipped at the front and each side, respectively. An inner stainless steel cap of the entry lock was sealed by a seamless silicone band. It was possible to ventilate this isolator by either free-flow or blower operation. After autoclaving the isolators 10 to 16 times for a year, none of them was repaired. Five mice can be kept in this isolator for about 1 month without supply of diet, water and wood shaving after the first setting.

摘要

设计了一种用于无菌小鼠实验的轻质不锈钢可高压灭菌五边形隔离器。该隔离器的腔室尺寸为400×450×350毫米,背面有一个直径200毫米的入口,天花板上有一扇窗户。前面和两侧分别装有玻璃罩和两个过滤器。入口锁的内部不锈钢盖由无缝硅胶带密封。该隔离器可以通过自由流动或鼓风机操作进行通风。一年中对这些隔离器进行10至16次高压灭菌后,没有一个需要维修。首次放置后,五只小鼠可以在这个隔离器中饲养约1个月,无需供应食物、水和木屑。

相似文献

1
[An autoclavable stainless steel isolator for small scale gnotobiotic experiments (author's transl)].用于小规模悉生生物学实验的可高压灭菌不锈钢隔离器(作者译)
Jikken Dobutsu. 1978 Jan;27(1):13-6. doi: 10.1538/expanim1978.27.1_13.
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An autoclavable isolator for operating on the adult gnotobiotic dog.一种用于成年无菌犬手术的可高压灭菌隔离器。
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A combination sterilizing chamber and transfer and housing isolator for use in gnotobiotic laboratories.一种用于悉生生物学实验室的组合式消毒室以及转移和容纳隔离器。
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A lightweight autoclavable free-flow germfree mouse isolator.一种轻便的可高压灭菌的自由流动无菌小鼠隔离器。
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