缺乏复制能力和产生XC噬斑能力的内源性亲嗜性小鼠C型病毒。
Endogenous ecotropic mouse type C viruses deficient in replication and production of XC plaques.
作者信息
Rapp U R, Nowinski R C
出版信息
J Virol. 1976 May;18(2):411-7. doi: 10.1128/JVI.18.2.411-417.1976.
Abstract
Endogenous ecotropic type C viruses were induced by iodedeoxyuridine from nontransformed and chemically or spontaneously transformed clones of the C3H/10T1/2 cell line. Viruses produced by cells of certain transformed clones were N-tropic and formed large XC plaques. In contrast, viruses produced by nontransformed C3H/10T1/2 cells were not detectable in the XC plaque test. These XC- viruses infected mouse cells with high efficiency, as shown by the induction of murine leukemia virus group-specific antigens in infected cells, but virus production, as determined by DNA polymerase-containing particles, was extremely low. Upon growth in certain mouse cells these replication-deficient, XC(-) viruses converted to type C viruses that were similar in XC assays to N-tropic AKR virus (XC+).
摘要
用碘脱氧尿苷从C3H/10T1/2细胞系的未转化以及化学转化或自发转化的克隆中诱导出内源性嗜亲性C型病毒。某些转化克隆的细胞产生的病毒是N嗜性的，并形成大的XC蚀斑。相比之下，未转化的C3H/10T1/2细胞产生的病毒在XC蚀斑试验中无法检测到。这些XC-病毒能高效感染小鼠细胞，如在感染细胞中诱导出鼠白血病病毒群特异性抗原来证明，但通过含DNA聚合酶颗粒测定的病毒产生量极低。在某些小鼠细胞中生长时，这些复制缺陷型的XC(-)病毒转变为C型病毒，在XC检测中与N嗜性AKR病毒（XC+）相似。
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