大鼠胚胎培养物中内源性亲嗜性C型病毒的自发释放。
Spontaneous release of endogenous ecotropic type C virus from rat embryo cultures.
作者信息
Rasheed S, Bruszewski J, Rongey R, Roy-Burman P, Charman H P, Gardner M B
出版信息
J Virol. 1976 May;18(2):799-803. doi: 10.1128/JVI.18.2.799-803.1976.
Type C viruses were isolated from embryo cultures of two different rat strains, Sprague-Dawley and Fischer. Both viruses (termed rat leukemia virus, RaLV) were released spontaneously from rat embryo cells, have a density of 1.14 to 1.15 g/cm(3) based on equilibrium sedimentation in sucrose gradients, contain 60-70S RNA, RNA-directed DNA polymerase, and rat type C virus-specific 30,000 molecular-weight-protein determinants. Molecular hybridization studies using the Sprague-Dawley RaLV 60-70S RNA show that the virus-specific nucleotide sequences are present in the DNA of rat embryos. Both Sprague-Dawley and Fischer RaLV can rescue the murine sarcoma virus genome from Kirsten murine sarcoma virus-transformed nonproducer cells and are neutralized by antisera to the RPL strain of RaLV. In contrast to previous RaLV's, these viruses propagate in their own cells of origin as well as in cells of heterologous rat strains.
C型病毒是从两种不同品系的大鼠（斯普拉格-道利大鼠和费希尔大鼠）的胚胎培养物中分离出来的。两种病毒（称为大鼠白血病病毒，RaLV）均从大鼠胚胎细胞中自发释放，根据在蔗糖梯度中的平衡沉降法，其密度为1.14至1.15 g/cm³，含有60 - 70S RNA、RNA指导的DNA聚合酶以及大鼠C型病毒特异性的30,000分子量蛋白质决定簇。使用斯普拉格-道利大鼠RaLV 60 - 70S RNA进行的分子杂交研究表明，病毒特异性核苷酸序列存在于大鼠胚胎的DNA中。斯普拉格-道利大鼠和费希尔大鼠的RaLV都能从克尔斯滕小鼠肉瘤病毒转化的非生产性细胞中拯救小鼠肉瘤病毒基因组，并被针对RaLV的RPL株的抗血清中和。与之前的RaLV不同，这些病毒在其自身的起源细胞以及异源大鼠品系的细胞中都能增殖。
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