炎性渗出物中抗炎因子的部分纯化
Partial purification of the anti-inflammatory factor(s) in inflammatory exudate.
作者信息
Billingham M E, Robinson B V, Robson J M
出版信息
Br J Pharmacol. 1969 Mar;35(3):543-57. doi: 10.1111/j.1476-5381.1969.tb08295.x.
Abstract
- The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti-inflammatory activity of inflammatory exudates.2. Incubation alone at a temperature above 70 degrees C or with pronase at 37 degrees C destroyed the anti-inflammatory activity of exudate.3. The anti-inflammatory component of exudate was partially precipitated by 50% ammonium sulphate.4. A partial purification process was devised using Sephadex G-150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification.5. Measurements of 11-hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its anti-inflammatory effects.
摘要
角叉菜胶足部试验被确立为一种用于测定炎症渗出物抗炎活性的灵敏且可靠的测定方法。
仅在70摄氏度以上温度孵育或在37摄氏度下用链霉蛋白酶处理会破坏渗出物的抗炎活性。
渗出物的抗炎成分可被50%硫酸铵部分沉淀。
设计了一种使用葡聚糖G - 150凝胶过滤以及DEAE和CM纤维素离子交换色谱的部分纯化方法，以实现至少24倍的纯化。
11 - 羟基皮质类固醇水平的测量表明，类固醇不参与渗出物产生抗炎作用的机制。
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