Klemm W, Liebich H M, Gupta D
J Clin Endocrinol Metab. 1976 Mar;42(3):514-9. doi: 10.1210/jcem-42-3-514.
A radioimmunoassay using as antibody against testosterone-3-carboxymethyloxime-BSA is described for the measurement of androstanediol (5alpha-androstane-3alpha,17beta-diol) in human plasma. Sephadex LH 20-column chromatography was used to separate other steroids croos-reaching with the antibody from androstanediol. The sensitivity of the assay was 7 pg and the recovery of labelled androstanediol was 65.1%. The intra-assay coefficient of variation was 9.9%. The existence of 5alpha-androstane-3alpha,17beta-diol in the androstanediol fraction could be demonstrated by gas chromatography and mass spectrometry (GC-MS). Plasma concentration of this substance was measured in 53 normally developing pre-pubertal and pubertal boys and in 13 adult men. The mean concentrations significantly rose from puberty stage 1 to 2, and stage 2 to 3. Although there was no significant difference between the plasma concentrations at stages 3 and 4, an increment from stage 4 and 5 was highly significant. Levels in adult males were significantly higher than those in the stage 5 of normal puberty.
本文描述了一种使用抗睾酮-3-羧甲基肟-BSA抗体的放射免疫分析法，用于测定人血浆中的雄烷二醇（5α-雄烷-3α,17β-二醇）。采用葡聚糖凝胶LH 20柱色谱法将与抗体发生交叉反应的其他类固醇与雄烷二醇分离。该分析方法的灵敏度为7皮克，标记雄烷二醇的回收率为65.1%。批内变异系数为9.9%。雄烷二醇组分中5α-雄烷-3α,17β-二醇的存在可通过气相色谱和质谱法（GC-MS）证实。对53名正常发育的青春期前和青春期男孩以及13名成年男性的该物质血浆浓度进行了测定。平均浓度从青春期1期到2期、2期到3期显著升高。虽然3期和4期的血浆浓度无显著差异，但从4期到5期有显著升高。成年男性的水平显著高于正常青春期5期的水平。
