呼肠孤病毒亚病毒颗粒在干扰素处理的小鼠L细胞中的形成及特性
Formation and characteristics of reovirus subviral particles in interferon-treated mouse L cells.
作者信息
Galster R L, Lengyel P
出版信息
Nucleic Acids Res. 1976 Mar;3(3):581-98. doi: 10.1093/nar/3.3.581.
We examined the early phase of reovirus replication in interferon-treated L cells. No difference was detected in the adsorption of virus to cells, the rate and extent of conversion of parental virions to sub-viral particles (SVPs), and the protein and double-stranded RNA composition of the SVPs when comparing reovirus infection of the interferon-treated and control cells. Furthermore, when tested in vitro, SVPs isolated from interferon-treated cells (SVPINT) synthesized and methylated reo mRNAs at the same rate as SVPs isolated from control cells (SVPCON). However, the accumulated products of RNA synthesis promoted by SVPCON consisted mainly of full size reo mRNA molecules, whereas those whose synthesis was promoted by SVPINT consisted mainly of shorter products. These results indicate that premature termination of transcription and/or degradation of full size transcripts occurred in vitro with SVPINT. Other experiments revealed that a nuclease is associated with our SVPINT preparation.
我们研究了呼肠孤病毒在干扰素处理的L细胞中的早期复制阶段。在比较干扰素处理细胞和对照细胞的呼肠孤病毒感染时，未检测到病毒对细胞的吸附、亲代病毒粒子向亚病毒颗粒（SVPs）转化的速率和程度以及SVPs的蛋白质和双链RNA组成存在差异。此外，在体外测试时，从干扰素处理细胞中分离的SVPs（SVPINT）合成和甲基化呼肠孤病毒mRNA的速率与从对照细胞中分离的SVPs（SVPCON）相同。然而，由SVPCON促进的RNA合成积累产物主要由全长呼肠孤病毒mRNA分子组成，而由SVPINT促进合成的产物主要由较短的产物组成。这些结果表明，在体外，SVPINT会发生转录过早终止和/或全长转录本的降解。其他实验表明，一种核酸酶与我们的SVPINT制剂相关。
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