Mäkelä O, Mitchison N A
Immunology. 1965 Jun;8(6):539-48.
The use of bacteriophage and bovine serum albumin as antigens for adoptive immunization has been investigated. Cells were transplanted from normal or previously immunized (`primed cells') donor mice into isologous hosts, and then stimulated with antigen. The reactions to phage of normal and primed cells could be distinguished and measured; for BSA, only primed cells could be used without the help of adjuvant. Irradiation of the prospective host enhanced the activity of the transplanted cells. Saturation was easily reached by increasing the number of phage-primed cells in the transplant. BSA-primed cells saturated less easily, probably because BSA is a relatively poor immunogen. Primed cells were localized, to a limited extent, in the lymph nodes draining the sites of injection of antigen, but could usually also be found in the blood.
已经研究了使用噬菌体和牛血清白蛋白作为过继免疫抗原的情况。将细胞从正常或先前免疫过的（“致敏细胞”）供体小鼠移植到同源宿主中，然后用抗原刺激。正常细胞和致敏细胞对噬菌体的反应可以区分和测量；对于牛血清白蛋白，在没有佐剂帮助的情况下只能使用致敏细胞。对预期宿主进行照射可增强移植细胞的活性。通过增加移植中噬菌体致敏细胞的数量很容易达到饱和。牛血清白蛋白致敏细胞不太容易达到饱和，可能是因为牛血清白蛋白是一种相对较差的免疫原。致敏细胞在一定程度上定位于引流抗原注射部位的淋巴结中，但通常也能在血液中发现。
