de Louvois J, Hurley R
Br Med J. 1977 Apr 16;1(6067):998-1000. doi: 10.1136/bmj.1.6067.998.
Four of 22 specimens of human pus inactivated up to 90% of added penicillin within one hour in vitro. Ampicillin and cephaloridine were also inactivated, but streptomycin and fusidic acid were not. The effect was not related to the protein content of the pus, nor to its pH value. Microbes that may produce beta-lactamase in small quantities were isolated from three of the four specimens, but the enzyme was not detected in the pus by physical methods nor by microbiological inhibition assay. The inactivating effect was shown to be a property of the solid portion of the pus, and was absent from the filtrate. We suggest that the effect may be an intrinsic property of the host, which should be investigated further as it has important implications for clinical practice.
22份人脓液标本中有4份在体外1小时内可使添加的青霉素失活达90%。氨苄西林和头孢菌素也会失活，但链霉素和夫西地酸不会。这种作用与脓液的蛋白质含量及其pH值均无关。从4份标本中的3份分离出了可能少量产生β-内酰胺酶的微生物，但通过物理方法或微生物抑制试验在脓液中均未检测到该酶。失活作用显示是脓液固体部分的特性，滤液中不存在这种作用。我们认为这种作用可能是宿主的一种内在特性，鉴于其对临床实践有重要意义，应进一步研究。
