Palmano K P, Whiting P H, Hawthorne J N
Biochem J. 1977 Oct 1;167(1):229-35. doi: 10.1042/bj1670229.
Acute diabetes with ketosis was induced in rats by intraperitoneal streptozotocin and also a milder form of diabetes without ketosis by injecting less of the drug. The acutely diabetic rats were killed 72h after injection and the others after either 2 or 13 weeks. Free and lipid myo-inositol was then measured in various tissues and body fluids by g.l.c. of the trimethylsilyl ether. Serum inositol was increased in the acutely diabetic group, whereas liver inositol was decreased. Brain and kidney inositol concentrations were increased in the mildly diabetic animals at 13 weeks and there was a progressive decrease in sciatic-nerve inositol. Lipid inositol of sciatic nerve was decreased in the acutely diabetic group only. Brain lipid inositol concentration was decreased in mild diabetes at 13 weeks. Possible implications of these findings in relation to diabetic neuropathy was discussed.
通过腹腔注射链脲佐菌素在大鼠中诱导出伴有酮症的急性糖尿病，通过注射较少剂量的该药物诱导出较轻的无酮症糖尿病形式。急性糖尿病大鼠在注射后72小时处死，其他大鼠在2周或13周后处死。然后通过三甲基硅醚的气相色谱法测量各种组织和体液中的游离和脂质肌醇。急性糖尿病组血清肌醇增加，而肝脏肌醇减少。轻度糖尿病动物在13周时脑和肾肌醇浓度增加，坐骨神经肌醇逐渐减少。仅急性糖尿病组坐骨神经的脂质肌醇减少。轻度糖尿病在13周时脑脂质肌醇浓度降低。讨论了这些发现与糖尿病神经病变相关的可能意义。
