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相似文献

1
Some parameters relevant to affinity chromatography on immobilized nucleotides.一些与固定化核苷酸亲和色谱相关的参数。
Biochem J. 1973 Jul;133(3):499-506. doi: 10.1042/bj1330499.
2
The selective retardation of NADP+-dependent dehydrogenases by immobilized procion red HE-3B.固定化普施安红HE - 3B对NADP + 依赖性脱氢酶的选择性抑制作用
Biochem J. 1978 Aug 1;173(2):591-6. doi: 10.1042/bj1730591.
3
General ligands in affinity chromatography. Cofactor-substrate elution of enzymes bound to the immobilized nucleotides adenosine 5'-monophosphate and nicotinamide-adenine dinucleotide.亲和色谱中的通用配体。固定化核苷酸5'-单磷酸腺苷和烟酰胺腺嘌呤二核苷酸结合的酶的辅因子-底物洗脱。
Biochem J. 1972 May;127(4):625-31. doi: 10.1042/bj1270625.
4
Affinity chromatography of nicotinamide nucleotide-dependent dehydrogenases on immobilized nucleotide derivatives.固定化核苷酸衍生物上烟酰胺核苷酸依赖性脱氢酶的亲和层析
Biochem J. 1974 Sep;141(3):775-87. doi: 10.1042/bj1410775.
5
Affinity chromatography of nicotinamide-adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide.烟酰胺腺嘌呤二核苷酸连接的脱氢酶在二核苷酸固定化衍生物上的亲和层析
Biochem J. 1973 Dec;135(4):595-607. doi: 10.1042/bj1350595.
6
Simple chemical synthesis of a specific effector for the affinity chromatography of nicotinamide adenine dinucleotide phosphate-dependent dehydrogenases.
Ital J Biochem. 1974 Sep-Oct;23(5):279-91.
7
Immobilized nucleotides and their use in affinity chromatography.固定化核苷酸及其在亲和色谱中的应用。
Int J Biochem. 1978;9(7):449-56. doi: 10.1016/0020-711x(78)90074-5.
8
The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.基于固定化辅因子的烟酰胺核苷酸依赖性脱氢酶的纯化
Biochem J. 1973 Jul;133(3):507-13. doi: 10.1042/bj1330507.
9
Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.适用于亲和色谱的腺苷核苷酸衍生物的制备。
Biochem J. 1974 Jun;139(3):609-23. doi: 10.1042/bj1390609.
10
Affinity chromatography of lactate dehydrogenase on immobilized nucleotides.乳酸脱氢酶在固定化核苷酸上的亲和层析
Biochem J. 1973 Jul;133(3):515-20. doi: 10.1042/bj1330515.

引用本文的文献

1
Nucleoside Scaffolds and Carborane Clusters for Boron Neutron Capture Therapy: Developments and Future Perspective.核苷骨架和碳硼烷簇用于硼中子俘获治疗：发展与未来展望。
Curr Med Chem. 2024;31(35):5739-5754. doi: 10.2174/0109298673245020230929152030.
2
N3-substituted thymidine bioconjugates for cancer therapy and imaging.用于癌症治疗和成像的 N3-取代的胸苷生物缀合物。
Future Med Chem. 2013 Apr;5(6):677-92. doi: 10.4155/fmc.13.31.
3
Some general methods of preparing affinity columns.制备亲和柱的一些常规方法。
Nucleic Acids Res. 1974 Nov;1(11):1587-99. doi: 10.1093/nar/1.11.1587.
4
The study of ligand-protein interactions utilizing affinity chromatography.利用亲和色谱法研究配体-蛋白质相互作用。
Appl Biochem Biotechnol. 1984 Jun;9(3):261-84. doi: 10.1007/BF02798492.
5
Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.适用于亲和色谱的腺苷核苷酸衍生物的制备。
Biochem J. 1974 Jun;139(3):609-23. doi: 10.1042/bj1390609.
6
Affinity chromatography of lactate dehydrogenase on immobilized nucleotides.乳酸脱氢酶在固定化核苷酸上的亲和层析
Biochem J. 1973 Jul;133(3):515-20. doi: 10.1042/bj1330515.
7
The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.基于固定化辅因子的烟酰胺核苷酸依赖性脱氢酶的纯化
Biochem J. 1973 Jul;133(3):507-13. doi: 10.1042/bj1330507.
8
An oligodeoxynucleotide affinity column for the isolation of sequence specific DNA binding proteins.用于分离序列特异性DNA结合蛋白的寡脱氧核苷酸亲和柱。
Nucleic Acids Res. 1988 Nov 11;16(21):10283-99. doi: 10.1093/nar/16.21.10283.
9
The purification of human enterokinase by affinity chromatography and immunoadsorption. Some observations on its molecular characteristics and comparisons with the pig enzyme.通过亲和色谱法和免疫吸附法纯化人肠激酶。关于其分子特性的一些观察以及与猪肠激酶的比较。
Biochem J. 1976 May 1;155(2):243-54. doi: 10.1042/bj1550243.
10
The effect of matrix on the binding of albumin to immobilized Cibacron Blue.基质对白蛋白与固定化汽巴蓝结合的影响。
Biochem J. 1977 Oct 1;167(1):301-3. doi: 10.1042/bj1670301.

本文引用的文献

1
Affinity chromatography of enzymes on insolubilized cofactors.
FEBS Lett. 1971 May 20;14(5):313-316. doi: 10.1016/0014-5793(71)80288-0.
2
Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
Nature. 1967 Jun 24;214(5095):1302-4. doi: 10.1038/2141302a0.
3
A micro phosphate method.
Anal Biochem. 1968 Dec;26(3):364-8. doi: 10.1016/0003-2697(68)90197-8.
4
Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers.酶与卤化氰活化多糖载体的化学固定
Eur J Biochem. 1971 Feb 1;18(3):351-60. doi: 10.1111/j.1432-1033.1971.tb01250.x.
5
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
6
Affinity chromatography: large-scale purification of the soluble oestradiol-17- dehydrogenase of human placenta.
FEBS Lett. 1972 Jun 15;23(2):175-9. doi: 10.1016/0014-5793(72)80334-x.
7
The mechanism of wall synthesis in bacteria. The organization of enzymes and isoprenoid phosphates in the membrane.细菌细胞壁合成的机制。膜中酶和类异戊二烯磷酸酯的组织。
Biochem J. 1972 Mar;127(1):11-25. doi: 10.1042/bj1270011.
8
Some applications of insolubilised cofactors to the purification of pyridine nucleotide-dependent dehydrogenases.不溶性辅因子在吡啶核苷酸依赖性脱氢酶纯化中的一些应用。
Biochem Biophys Res Commun. 1972 Aug 21;48(4):1004-10. doi: 10.1016/0006-291x(72)90708-5.
9
Affinity chromatography.亲和层析
Annu Rev Biochem. 1971;40:259-78. doi: 10.1146/annurev.bi.40.070171.001355.
10
Preparation of a NAD(H)-polymer matrix showing coenzyme function of the bound pyridine nucleotide.一种显示结合的吡啶核苷酸辅酶功能的NAD(H) -聚合物基质的制备。
Biotechnol Bioeng. 1971 May;13(3):393-8. doi: 10.1002/bit.260130306.

一些与固定化核苷酸亲和色谱相关的参数。

Some parameters relevant to affinity chromatography on immobilized nucleotides.

作者信息

Lowe C R, Harvey M J, Craven D B, Dean P D

出版信息

Biochem J. 1973 Jul;133(3):499-506. doi: 10.1042/bj1330499.

DOI:10.1042/bj1330499
PMID:4354739
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177727/
Abstract
  1. The suitability of cellulose and Sepharose as supports for affinity chromatography of two groups of cofactor-linked enzymes, dehydrogenases and kinases, was examined. Sepharose was found to be superior. 2. The selective capacities of the columns were measured by frontal analysis and are discussed in relation to the nucleotide contents. 3. The effect of various concentrations of enzyme and of non-specific protein on the performance of the affinity columns, and the effects of equilibration time, flow rate, sample volume and dilution of the nucleotide were examined. 4. The effect of interposing polymethylene and polyglycine extension arms between the matrix backbone and the nucleotide was investigated for several cofactor-dependent enzymes. Maximum binding was observed with an extension arm 0.8-1nm long.
摘要
  1. 研究了纤维素和琼脂糖作为两类与辅因子相连的酶（脱氢酶和激酶）亲和色谱支持物的适用性。结果发现琼脂糖更具优势。2. 通过前沿分析法测定了柱的选择性容量，并结合核苷酸含量进行了讨论。3. 考察了不同浓度的酶和非特异性蛋白质对亲和柱性能的影响，以及平衡时间、流速、样品体积和核苷酸稀释的影响。4. 针对几种依赖辅因子的酶，研究了在基质主链和核苷酸之间插入亚甲基和聚甘氨酸延伸臂的效果。观察到延伸臂长度为0.8 - 1纳米时结合力最大。