[用于在角质层制剂、低温恒温器切片和简单冷冻切片中显示生物胺的乙醛酸荧光法的经验]
[Experiences with the glyoxylic-acid fluorescence method for the demonstration of biogenic amines in cuticle preparations, cryostat- and simple frozen sections].
作者信息
Stach W, Jonas L
出版信息
Z Mikrosk Anat Forsch. 1976;90(6):1041-8.
PMID:801849
Abstract
- Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--incubation of fresh tissue in glyoxylic acid of 1...2% (pH-value 7.0)--making ready whole mounts, cryostat-or simple frozen sections--air-drying--exposure to 80...100 degrees C for 10...3 minutes--embedding with paraffinum subliquidum. Fluorescence microscopy and photography may be done in the bright and dark field, as well with usual filter-combinations.
摘要
- 我们的研究旨在进行方法上的简化，以使乙醛酸荧光法可供所有人使用。我们成功地在整装片、低温恒温器切片和简单冷冻切片的情况下获得了足够的结果。2. 制备载玻片标本可分为5个步骤：——将新鲜组织在1...2%的乙醛酸（pH值7.0）中孵育——制备整装片、低温恒温器切片或简单冷冻切片——空气干燥——在80...100摄氏度下暴露10...3分钟——用液体石蜡包埋。荧光显微镜检查和摄影可在明场和暗场进行，也可使用常规滤光片组合。
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