[Experiences with the glyoxylic-acid fluorescence method for the demonstration of biogenic amines in cuticle preparations, cryostat- and simple frozen sections].

1. Our studies aimed at methodical simplification to make available the glyoxylic acid-fluorescence method to everybody. We succeeded in receiving adequate results in the case of whole mounts, cryostat- and simple frozen sections. 2. Making ready the slide preparations may be divided into 5 steps:--incubation of fresh tissue in glyoxylic acid of 1...2% (pH-value 7.0)--making ready whole mounts, cryostat-or simple frozen sections--air-drying--exposure to 80...100 degrees C for 10...3 minutes--embedding with paraffinum subliquidum. Fluorescence microscopy and photography may be done in the bright and dark field, as well with usual filter-combinations.