粗糙脉孢菌中的钴毒性与铁代谢

Padmanaban G, Sarma P S

Biochem J. 1966 Jan;98(1):330-4. doi: 10.1042/bj0980330.

Increasing concentrations of cobalt in the medium result in increased production of an iron-binding compound and a corresponding fall in catalase activity of Neurospora crassa. 2. Cobalt rapidly depletes the medium of iron by enhancing the rate of iron uptake by the mycelium. 3. With toxic amounts of cobalt there is a fall in bound (59)Fe and haem (59)Fe as well as a decreased incorporation of [2-(14)C]glycine into the mycelial haem fraction. The production of the iron-binding compound precedes the fall in the iron-dependent systems mentioned. 4. The (59)Fe bound to the iron-binding compound acts as a better iron source for haem synthesis in cell-free extracts as compared with (59)FeSO(4). 5. Cobalt inhibits iron incorporation into protoporphyrin in cell-free extracts but is not itself incorporated to an appreciable extent.

培养基中钴浓度的增加会导致一种铁结合化合物的产量增加，同时粗糙脉孢菌的过氧化氢酶活性相应下降。2. 钴通过提高菌丝体对铁的摄取速率，迅速耗尽培养基中的铁。3. 在含有毒性剂量钴的情况下，结合态（59）铁和血红素（59）铁含量下降，同时[2-（14）C]甘氨酸掺入菌丝体血红素部分的量也减少。铁结合化合物的产生先于上述铁依赖系统的下降。4. 与（59）硫酸亚铁相比，结合在铁结合化合物上的（59）铁在无细胞提取物中作为血红素合成的铁源效果更好。5. 钴在无细胞提取物中抑制铁掺入原卟啉，但钴本身掺入的程度不明显。

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核心技术专利：CN118964589B侵权必究

Padmanaban G, Sarma P S

Biochem J. 1966 Jan;98(1):330-4. doi: 10.1042/bj0980330.

Increasing concentrations of cobalt in the medium result in increased production of an iron-binding compound and a corresponding fall in catalase activity of Neurospora crassa. 2. Cobalt rapidly depletes the medium of iron by enhancing the rate of iron uptake by the mycelium. 3. With toxic amounts of cobalt there is a fall in bound (59)Fe and haem (59)Fe as well as a decreased incorporation of [2-(14)C]glycine into the mycelial haem fraction. The production of the iron-binding compound precedes the fall in the iron-dependent systems mentioned. 4. The (59)Fe bound to the iron-binding compound acts as a better iron source for haem synthesis in cell-free extracts as compared with (59)FeSO(4). 5. Cobalt inhibits iron incorporation into protoporphyrin in cell-free extracts but is not itself incorporated to an appreciable extent.

培养基中钴浓度的增加会导致一种铁结合化合物的产量增加，同时粗糙脉孢菌的过氧化氢酶活性相应下降。2. 钴通过提高菌丝体对铁的摄取速率，迅速耗尽培养基中的铁。3. 在含有毒性剂量钴的情况下，结合态（59）铁和血红素（59）铁含量下降，同时[2-（14）C]甘氨酸掺入菌丝体血红素部分的量也减少。铁结合化合物的产生先于上述铁依赖系统的下降。4. 与（59）硫酸亚铁相比，结合在铁结合化合物上的（59）铁在无细胞提取物中作为血红素合成的铁源效果更好。5. 钴在无细胞提取物中抑制铁掺入原卟啉，但钴本身掺入的程度不明显。