填充床中羧甲基纤维素 - 无花果蛋白酶的动力学
The kinetics of carboxymethylcellulose--ficin in packed beds.
作者信息
Lilly M D, Hornby W E, Crook E M
出版信息
Biochem J. 1966 Sep;100(3):718-23. doi: 10.1042/bj1000718.
Abstract
- The kinetics of the hydrolysis of benzoylarginine ethyl ester in packed columns of CM-cellulose-70-ficin and CM-cellulose-90-ficin were studied. 2. The apparent Michaelis constant, K'(m), of these preparations was calculated and shown to be dependent on the flow rate at low rates of perfusion through the columns. 3. The values for k(3) of these preparations were calculated and shown to be nearly independent of flow rate. 4. A modified form of the integrated Michaelis rate equation was used to describe the action of these materials and its limitations are discussed. 5. The hydrolysis of solutions of casein by these columns was studied.
摘要
- 研究了苯甲酰精氨酸乙酯在CM - 纤维素 - 70 - 无花果蛋白酶和CM - 纤维素 - 90 - 无花果蛋白酶填充柱中的水解动力学。2. 计算了这些制剂的表观米氏常数K'(m)，结果表明在低灌注速率下通过柱子时，其依赖于流速。3. 计算了这些制剂的k(3)值，结果表明其几乎与流速无关。4. 使用了积分米氏速率方程的修正形式来描述这些材料的作用，并讨论了其局限性。5. 研究了这些柱子对酪蛋白溶液的水解作用。
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2
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