Sabet T Y, Friedman H
Immunology. 1970 Nov;19(5):843-53.
The effects of colloidal carbon particles on induction of antibody-forming cells in tissue-culture suspensions of spleen cells from normal or sheep erythrocyte primed donor mice were determined. Addition of carbon to normal spleen-cell cultures prevented induction of the primary' type immune response . The time at which the carbon was added to the cultures was important, since less suppression occurred when carbon treatment was delayed 48 hours after initiation of culture. Furthermore, the most suppression occurred with the largest dose of carbon used. Injection of normal donor mice with carbon at various time intervals prior to death interfered with the ability of the spleen-cell suspensions to respond to sheep erythrocytes by formation of antibody plaques. Maximum suppression occurred when the donor mice were treated with carbon 1–2 days before they were killed. The anamnestic antibody plaque response was also suppressed by addition of carbon particles to spleen-cell cultures derived from mice primed with sheep erythrocytes several weeks before they were killed. There was a greater suppression of the 7S than 19S PFC response . However, the only marked suppression occurred when the highest dose of carbon was used and when it was added to the cultures at 0' time. Direct injection of carbon into mice primed with sheep erythrocytes 3–4 weeks previously also interfered with the secondary response of the spleen-cell cultures . Both 19S and 7S antibody-forming cells were suppressed, with a greater suppression of 7S PFCs. The results of these experiments were interpreted as indicating that phagocytic cells, capable of being `blockaded' or by carbon suspensions, are necessary for induction of both primary and secondary type antibody plaque responses . It seems likely that the carbon particles compete with the red blood cell antigens for a limited number of antigen-processing cells.
测定了胶体碳颗粒对来自正常或经绵羊红细胞致敏的供体小鼠脾细胞组织培养悬液中抗体形成细胞诱导的影响。向正常脾细胞培养物中添加碳可阻止“初次”型免疫应答的诱导。向培养物中添加碳的时间很重要，因为在培养开始后延迟48小时进行碳处理时抑制作用较小。此外，使用的碳剂量越大，抑制作用越强。在处死前不同时间间隔给正常供体小鼠注射碳会干扰脾细胞悬液通过形成抗体斑对绵羊红细胞作出反应的能力。当供体小鼠在处死前1 - 2天接受碳处理时，抑制作用最大。向处死前几周用绵羊红细胞致敏的小鼠的脾细胞培养物中添加碳颗粒也会抑制回忆性抗体斑反应。7S PFC反应比19S PFC反应受到的抑制更大。然而，只有在使用最高剂量的碳并在“0”时间添加到培养物中时才会出现明显的抑制作用。直接向3 - 4周前用绵羊红细胞致敏的小鼠注射碳也会干扰脾细胞培养物的二次反应。19S和7S抗体形成细胞均受到抑制，7S PFCs受到的抑制更大。这些实验结果被解释为表明能够被碳悬液“阻断”或吞噬的吞噬细胞对于初次和二次型抗体斑反应的诱导都是必需的。碳颗粒似乎与红细胞抗原竞争有限数量的抗原处理细胞。
