来自绳状青霉的一种抗病毒物质。VI. 海伦宁对小鼠感染辛德毕斯森林病毒后被动免疫建立的预防作用

An antiviral substance from Penicillium funiculosum. VI. Prevention of the establishment of passive immunity to Semliki Forest virus infection in mice by Helenine.

作者信息

Shope R E

出版信息

J Exp Med. 1966 Jul 1;124(1):15-31. doi: 10.1084/jem.124.1.15.

DOI:10.1084/jem.124.1.15

PMID:5944346

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2138221/

Abstract

Helenine prevents the establishment in mice of passive viral immunity by anti-Semliki serum of swine, rabbit, or guinea pig origin. 2. A period of 12 days must elapse, between the antiviral serum administration and challenge with virus, for prevention of the establishment of passive immunity to become apparent. This period is believed to correspond to that in which injected antibody persists in circulation in the injected host. 3. Helenine is effective in preventing the establishment of passive viral immunity by heterologous antiviral sera when it is administered any time during a period of 6 days, extending from 4 days before to 2 days after injection of the antiviral serum. 4. Helenine does not prevent the establishment of passive viral immunity by antiviral sera of mouse origin (homologous). 5. Evidence is presented to indicate that the phenomenon of the prevention of the establishment of passive viral immunity by heterologous antiviral sera is not effected directly, but rather is mediated through some substance that helenine induces the injected host to elaborate. 6. The capacity to prevent the establishment of passive viral immunity could not be exhausted by repeated preceding injections of helenine at 2- or 3-day intervals. 7. Evidence is presented to indicate that the helenine-induced material does not act upon antiviral antibody per se but rather on heterologous foreign protein that happens to be labeled as Semliki Forest virus antibody. This helenine-induced material, whatever its nature, appears to enhance the capacity of the injected host to recognize and dispose of foreign protein. 8. Statolon, a material that like helenine is a known inducer of interferon, is, like helenine, also capable of preventing the establishment of passive viral immunity by heterologous antiviral sera. 9. Experiments designed to determine whether the induced material responsible for the antipassive immunity effect of helenine is interferon have yielded inconclusive answers thus far.

摘要

海勒宁可阻止猪、兔或豚鼠源抗Semliki血清在小鼠体内建立被动病毒免疫。2. 在给予抗病毒血清和用病毒攻击之间必须经过12天，被动免疫的建立才能得到明显预防。这段时间被认为与注射的抗体在注射宿主循环中持续存在的时间相对应。3. 从注射抗病毒血清前4天到注射后2天的6天时间内，无论何时给予海勒宁，它都能有效阻止异源抗病毒血清建立被动病毒免疫。4. 海勒宁不能阻止小鼠源（同源）抗病毒血清建立被动病毒免疫。5. 有证据表明，异源抗病毒血清阻止被动病毒免疫建立的现象不是直接发生的，而是通过海勒宁诱导注射宿主产生的某种物质介导的。6. 以2天或3天的间隔重复预先注射海勒宁，不能耗尽阻止被动病毒免疫建立的能力。7. 有证据表明，海勒宁诱导的物质本身并不作用于抗病毒抗体，而是作用于碰巧被标记为Semliki森林病毒抗体的异源外来蛋白质。这种海勒宁诱导的物质，无论其性质如何，似乎都能增强注射宿主识别和处理外来蛋白质的能力。8. 斯他洛隆是一种与海勒宁一样已知的干扰素诱导剂，与海勒宁一样，也能够阻止异源抗病毒血清建立被动病毒免疫。9. 旨在确定负责海勒宁抗被动免疫作用的诱导物质是否为干扰素的实验，迄今为止尚未得出明确结论。