Maes R F, Vieira A, Gomes I, Auge de Mello P, Bernal Lopez C, Costa Freitas K
Arch Virol. 1977;55(4):275-85. doi: 10.1007/BF01315049.
The effect of various polycations on the immune response potentiated with poly I:C was studied. It was found that low molecular weight polycations had no potentiating effect. Polylysine was ineffective whereas protamine was superior to lysozyme, poly-arginine, poly-histidine, DEAE-Dextran and histone. A foot-and-mouth disease trivalent vaccine composed of strains A24 Cruzeiro, O1 Caseros and C2 Resende elicited no immune response in swine when adjuvanted with aluminium hydroxide but was effective when emulsified in oil. In general, the immune response was potentiated ten-fold when the emulsion contained poly I:C. The antibody production was in most cases further potentiated by a factor of ten when the nucleic acid double-strand was complexed with 1 : 10 (w/w) DEAE-Dextran. Protamine was as effective, or perhaps even more, than DEAE-Dextran to this effect. Guinea pigs vaccinated with a water-in-oil emulsion type monovalent C3 vaccine showed an increase in antibody production when the vaccine contained poly I:C or poly I:C complexed with 1 : 10 (w/w) protamine.
研究了各种聚阳离子对用聚肌胞苷酸（poly I:C）增强免疫反应的影响。发现低分子量聚阳离子没有增强作用。聚赖氨酸无效，而鱼精蛋白优于溶菌酶、聚精氨酸、聚组氨酸、二乙氨基乙基葡聚糖（DEAE - Dextran）和组蛋白。由A24 Cruzeiro株、O1 Caseros株和C2 Resende株组成的口蹄疫三价疫苗，用氢氧化铝佐剂时在猪中未引发免疫反应，但油包乳化时有效。一般来说，当乳剂含有聚肌胞苷酸时，免疫反应增强了10倍。在大多数情况下，当核酸双链与1:10（w/w）的二乙氨基乙基葡聚糖复合时，抗体产生进一步增强了10倍。在这种作用上，鱼精蛋白与二乙氨基乙基葡聚糖一样有效，甚至可能更有效。用油包水乳剂型单价C3疫苗接种的豚鼠，当疫苗含有聚肌胞苷酸或与1:10（w/w）鱼精蛋白复合的聚肌胞苷酸时，抗体产生增加。
