组蛋白H2A和H2B在染色质中沿DNA相邻：含H2A + H2B的亚核小体颗粒的特性
Histones H2A and H2B are neighbors along DNA in chromatin: characterization of subnucleosomal particles containing H2A+H2B.
作者信息
Nelson D A, Oosterhof D K, Rill R L
出版信息
Nucleic Acids Res. 1977 Dec;4(12):4223-34. doi: 10.1093/nar/4.12.4223.
Two specific slow sedimenting nucleoprotein particles containing equimolar amounts of histones H2A and H2B and 38 or 49 base pair (bp) lengths of DNA have been isolated by centrifugation on sucrose gradients. The 3.4S particles containing 38 bp DNA and H2A+H2B thermally denature at 61 degrees, considerably higher than Proteinase K treated particles (44 degrees), but lower than 11S nucleosomes (76 degrees). Treatment with Proteinase K increases the circular dichroism of 3.4S particles at 280 nm by 63% and decreases the sedimentation coefficient to 2.1S. These results indicate that H2A and H2B are proximate along DNA in nucleosomes and alone can alter the optical activity and perhaps conformation of local regions of DNA.
通过在蔗糖梯度上离心，已分离出两种特定的慢沉降核蛋白颗粒，它们含有等摩尔量的组蛋白H2A和H2B以及38或49个碱基对（bp）长度的DNA。含有38 bp DNA和H2A + H2B的3.4S颗粒在61摄氏度时发生热变性，大大高于经蛋白酶K处理的颗粒（44摄氏度），但低于11S核小体（76摄氏度）。用蛋白酶K处理会使3.4S颗粒在280 nm处的圆二色性增加63%，并使沉降系数降至2.1S。这些结果表明，在核小体中H2A和H2B沿DNA彼此靠近，并且单独就能改变DNA局部区域的光学活性以及可能的构象。
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