Cinader B, St Rose J E, Yoshimura M
J Exp Med. 1967 Jun 1;125(6):1057-73. doi: 10.1084/jem.125.6.1057.
Rabbits were rendered tolerant to human albumin (HA) and were then injected with azo and oxazolonated derivatives of human albumin. These injections were continued to a time at which all animals would have lost tolerance if they had not been injected. Injection of cross-reacting antigens prolonged the duration of tolerance, as judged by the mode of elimination of lightly iodinated human albumin (HA.(131)I). Different derivatives of HA differed in their capacity to prolong tolerance. Those neonatally injected rabbits which were immunized with cross-reacting antigens and lost tolerance, responded much more promptly to HA.(131)I than animals which were not immunized. Animals immunized with cross-reacting antigen which went on to eliminate HA.(131)I triphasically, usually had responded earlier by making antibodies. These antibodies contained a fraction which was reactive with HA, and which was usually equally well adapted to determinants on HA and on the cross-reacting antigen.
使兔子对人白蛋白（HA）产生耐受性，然后给它们注射人白蛋白的偶氮和恶唑酮化衍生物。这些注射持续进行到一个时间点，如果未进行注射，所有动物都会失去耐受性。通过轻度碘化人白蛋白（HA.(131)I）的消除方式判断，注射交叉反应抗原可延长耐受期。HA的不同衍生物在延长耐受的能力上有所不同。那些新生时注射过且用交叉反应抗原免疫后失去耐受性的兔子，对HA.(131)I的反应比未免疫的动物要迅速得多。用交叉反应抗原免疫并继而三相性消除HA.(131)I的动物，通常更早产生抗体。这些抗体含有一部分与HA反应的成分，并且通常同样能很好地适应HA和交叉反应抗原上的决定簇。
