Klein H P, Volkmann C M, Leaffer M A
J Bacteriol. 1967 Jul;94(1):61-5. doi: 10.1128/jb.94.1.61-65.1967.
When the crude ribosomal fraction of Saccharomyces cerevisiae was separated into "light" and "heavy" fractions, fatty acid synthetase was concentrated in the former, whereas acetyl-Coenzyme A synthetase, fatty acid "desaturase," and squalene oxidocyclase were found in the latter. The "desaturase" sedimented with the ribosomal material and was not solubilized by low concentrations of sodium deoxycholate (DOC). The other two systems found in the "heavy" fraction sedimented with the membranes, but, upon solubilization of the membranes by DOC, these enzyme systems remained as particles.
当将酿酒酵母的粗核糖体组分分离成“轻”组分和“重”组分时，脂肪酸合成酶集中在前一组分中，而乙酰辅酶A合成酶、脂肪酸“去饱和酶”和角鲨烯氧化环化酶则在后一组分中被发现。“去饱和酶”与核糖体物质一起沉淀，并且不被低浓度的脱氧胆酸钠（DOC）溶解。在“重”组分中发现的另外两个系统与膜一起沉淀，但是，当通过DOC使膜溶解时，这些酶系统仍以颗粒形式存在。
