Brand C M, Stealey V M, Rowe J
Dev Biol Stand. 1977;39:3-10.
The internal matrix proteins of A/Okuda/57, A/Finland/4/74 and A/New Jersey/8/76 viruses and several recombinant strains have been examined by radioiodination of the purified proteins followed by peptide mapping. The method is rapid and requires only small amounts of material. Reproducible differences were detected between the matrix proteins of the above parents and allowed the origin of the matrix proteins of the recombinant viruses to be determined. The use of matrix protein identity as a marker in recombination work and its possible correlation with biological properties of the virus is discussed.
通过对纯化后的蛋白质进行放射性碘化，随后进行肽图谱分析，对A/奥久田/57、A/芬兰/4/74和A/新泽西/8/76病毒以及几种重组毒株的内部基质蛋白进行了研究。该方法快速，且仅需少量材料。在上述亲本的基质蛋白之间检测到了可重复的差异，从而能够确定重组病毒基质蛋白的来源。本文讨论了将基质蛋白同一性用作重组研究中的标记及其与病毒生物学特性可能存在的相关性。
