Brand C M, Stealey V M, Rowe J
J Gen Virol. 1977 Sep;36(3):385-94. doi: 10.1099/0022-1317-36-3-385.
We have examined the matrix proteins of A/Okuda/57, A/Finland/4/74 and A/New Jersey/8/76 viruses and several recombinant strains by radioiodination of the purified polypeptides followed by tryptic peptide mapping. The method is rapid and requires only small amounts of material. Reproducible differences were detected between the matrix proteins of the above parents and allowed origin of the matrix proteins of the recombinant viruses to be determined. The possible use of matrix protein identity as a marker in recombination work is discussed.
我们通过对纯化的多肽进行放射性碘化，然后进行胰蛋白酶肽图谱分析，研究了A/奥田/57、A/芬兰/4/74和A/新泽西/8/76病毒以及几种重组毒株的基质蛋白。该方法快速，只需要少量材料。在上述亲本的基质蛋白之间检测到了可重复的差异，从而能够确定重组病毒基质蛋白的来源。文中还讨论了将基质蛋白同一性用作重组研究标记的可能性。
