Moriarty G C
J Histochem Cytochem. 1976 Jul;24(7):846-63. doi: 10.1177/24.7.60435.
The storage sites of the pituitary glycoprotein hormones were identified with the use of electron microscopic immunocytochemical techniques and antisera to the beta (beta) chains of follicle-stimulating hormone (FSH), luteinizing hormone (LH) and thyroid-stimulating hormone (TSH). The TSH cells in normal rats is ovoid or angular and contains small granules 60-160 nm in diameter. In TSH cells hypertrophied 45 days after thyroidectomy, staining is in globular patches in granules or diffusely distributed in the expanded profiles of dilated rough endoplasmic reticulum. The gonadotrophs (FSH and LH cells) exhibited three different morphologies. Type I cells are ovoid with a population of large granules and a population of small granules. Staining for FSHbeta or LHbeta was intense and specific only in the large granules (diameter of 400 nm or greater). Type II cells are angular or stellate and contain numerous secretory granules averaging 200-220 nm in diameter. They predominate during stages in the estrous cycle when FSH or LH secretion is high. Type III cells look like adrenocorticotropin (ACTH) cells in that they are stellate with peripherally arranged granules. They generally stain only with anti-FSHbeta and their staining can not be abolished by the addition of 100 ng ACTH. In preliminary quantitative studies of cycling females, we found that on serial sections FSH cells and LH cells show similar shifts to a more angular population of cells during stages of active secretion. However, the shifts are not in phase with one another. Furthermore, there are at least 1.5 times more FSH cells than LH cells at all stages of the cycle. Our collection of serial cells shows that some cells (usually type I or II) stain for both gonadotropic hormones, whereas others (usually type II or III) contain only one.
利用电子显微镜免疫细胞化学技术以及针对促卵泡激素（FSH）、促黄体生成素（LH）和促甲状腺激素（TSH）的β链的抗血清，确定了垂体糖蛋白激素的储存部位。正常大鼠的促甲状腺激素细胞呈卵圆形或角形，含有直径为60 - 160纳米的小颗粒。在甲状腺切除术后45天肥大的促甲状腺激素细胞中，染色呈颗粒状的球状斑块或弥散分布于扩张的粗面内质网的扩张轮廓中。促性腺激素细胞（FSH和LH细胞）表现出三种不同的形态。I型细胞呈卵圆形，有一群大颗粒和一群小颗粒。FSHβ或LHβ的染色仅在大颗粒（直径400纳米或更大）中强烈且特异。II型细胞呈角形或星状，含有许多平均直径为200 - 220纳米的分泌颗粒。在发情周期中FSH或LH分泌高的阶段，它们占主导。III型细胞看起来像促肾上腺皮质激素（ACTH）细胞，呈星状，颗粒排列在周边。它们通常仅用抗FSHβ染色，且添加100纳克ACTH不能消除其染色。在对处于发情周期的雌性大鼠的初步定量研究中，我们发现，在连续切片上，FSH细胞和LH细胞在活跃分泌阶段显示出向更角形的细胞群体的类似转变。然而，这些转变并不同步。此外，在周期的所有阶段，FSH细胞比LH细胞至少多1.5倍。我们收集的连续细胞显示，一些细胞（通常是I型或II型）对两种促性腺激素都染色，而另一些细胞（通常是II型或III型）只含有一种。
