Masihi K N, Werner H
Z Parasitenkd. 1977 Dec 29;54(3):209-16. doi: 10.1007/BF00390112.
Mice were infected with eleven cyst-forming Toxoplasma strains of varying virulence and challenged 1 month later with highly virulent BK strain parasites. The early cellular reaction in vivo was estimated by collecting the peritoneal exudates 24 h after challenge. This consisted of 54.34--77.61% lymphocytes and 0-8.88% infected macrophages in the eleven immunized groups in contrast to 27.17% lymphocytes and 18.64% infected macrophages in the control group. The peritoneal exudate 72 h after challenge comprised of 55.88-73.86% lymphocytes and 0-6.97% infected macrophages compared to 25.88% lymphocytes and 92% infected macrophages in the control group. Following the virulent challenge, deaths occurred in seven of the eleven groups immunized with live strains and ranged 5-42% at the end of a 6-week observation period. The most virulent of the Toxoplasma strains used - Alt and Gail - gave higher mortality while the least virulent ones - 558, 1070, K8 and KSU - were solidly resistant. The significantly different mortality obtained with strains Alt, Gail and Witting in a similar experiment performed at an interval of 7 months is discussed.
将小鼠感染11种不同毒力的形成包囊的弓形虫菌株，1个月后用高毒力的BK株寄生虫进行攻击。通过在攻击后24小时收集腹腔渗出液来评估体内早期细胞反应。在11个免疫组中，腹腔渗出液由54.34%至77.61%的淋巴细胞和0至8.88%的感染巨噬细胞组成，相比之下，对照组中淋巴细胞占27.17%，感染巨噬细胞占18.64%。攻击后72小时的腹腔渗出液中淋巴细胞占55.88%至73.86%，感染巨噬细胞占0至6.97%，而对照组中淋巴细胞占25.88%，感染巨噬细胞占92%。在强毒攻击后，11个用活菌株免疫的组中有7个组出现死亡，在6周观察期结束时死亡率在5%至42%之间。所用弓形虫菌株中毒力最强的——Alt和Gail——导致较高的死亡率，而毒力最弱的——558、1070、K8和KSU——则具有很强的抵抗力。讨论了在间隔7个月进行的类似实验中，Alt、Gail和Witting菌株获得的显著不同的死亡率。
