Schwab J H, Ohanian S H
J Bacteriol. 1967 Nov;94(5):1346-52. doi: 10.1128/jb.94.5.1346-1352.1967.
Specific chemical modification of group A polysaccharide antigen to the A-variant structure was demonstrated in the lymphoid organs of mice by autoradiography by use of radioantibodies specific for these structures. Both antigenic moieties persisted and were still discerned 10 weeks after injection of the group A cell wall. In rabbit skin, the group A specificity was altered after a prolonged period. Unlike the situation for the mouse, polysaccharide A was not converted to A-variant structure, but another specificity common to both polysaccharides persisted at the site of injection. Mucopeptide, separated from the polysaccharide of group A cell walls, was eliminated from the site of injection in rabbit skin between 4 and 8 hr after injection. Group D streptococcal cell walls were also rapidly eliminated from tissue, and were no longer detectable 8 hr after injection into rabbit skin or 24 hr after injection into mice. The rapid degradation of these structures was correlated with their susceptibility to lysozyme in vitro and was in contrast to the prolonged persistence of group A cell walls, which were completely resistant to egg white lysozyme. This persistence in tissue correlated with the capacity of group A cell wall fragments to induce a chronic inflammatory process, whereas the isolated mucopeptide or group D cell walls produced only an acute necrotoxic reaction.
通过使用针对这些结构的放射性抗体进行放射自显影，在小鼠的淋巴器官中证实了A群多糖抗原向A变异体结构的特异性化学修饰。两种抗原部分在注射A群细胞壁后10周仍持续存在且仍可辨别。在兔皮中，经过较长时间后A群特异性发生了改变。与小鼠的情况不同，多糖A未转化为A变异体结构，但两种多糖共有的另一种特异性在注射部位持续存在。从A群细胞壁多糖中分离出的粘肽在注射后4至8小时从兔皮注射部位清除。D群链球菌细胞壁也从组织中迅速清除，在注射到兔皮后8小时或注射到小鼠后24小时不再可检测到。这些结构的快速降解与其在体外对溶菌酶的敏感性相关，这与A群细胞壁的长期持续存在形成对比，A群细胞壁对蛋清溶菌酶完全有抗性。在组织中的这种持续存在与A群细胞壁片段诱导慢性炎症过程的能力相关，而分离出的粘肽或D群细胞壁仅产生急性坏死毒性反应。
