Abdulla E M, Schwab J H
J Bacteriol. 1966 Jan;91(1):374-83. doi: 10.1128/jb.91.1.374-383.1966.
Abdulla, Essa M. (University of North Carolina School of Medicine, Chapel Hill), and John H. Schwab. Biological properties of streptococcal cell-wall particles. III. Dermonecrotic reaction to cell-wall mucopeptides. J. Bacteriol. 91:374-383. 1966.-Intradermal injection of rabbits and guinea pigs with mucopeptide suspensions produced an acute necrotic lesion which reached maximal severity within 24 hr and gradually subsided with scar formation. Necrosis was evident within 4 hr after injection of 100 mug, and an indurated area (10 x 10 mm) was produced with as little as 5.0 mug. Mucopeptides from six bacterial strains were studied. Comparison of cell walls and derived mucopeptides showed that the acute necrotic lesion tended to be more severe as the residual polysaccharide was decreased. Hyperimmunization with mucopeptide reduced the acute reaction, with evidence of immunological specificity. Incubation with lysozyme also modified the reaction in relation to extent of digestion. Toxicity was related to particle size, since extended sonic vibration decreased activity. Histological sections showed intense accumulations of polymorphonuclear leukocytes, along with altered collagen. A chronic nodular lesion appeared about 7 days after injection of the intact cell-wall fragments. In contrast to the acute necrotic reaction, this lesion was rarely produced by the mucopeptide separated from polysaccharide.
阿卜杜拉，埃萨·M.（北卡罗来纳大学医学院，教堂山），以及约翰·H.施瓦布。链球菌细胞壁颗粒的生物学特性。III. 对细胞壁粘肽的皮肤坏死反应。《细菌学杂志》91:374 - 383。1966年。——给兔子和豚鼠皮内注射粘肽悬浮液会产生急性坏死性病变，该病变在24小时内达到最大严重程度，随后逐渐消退并形成瘢痕。注射100微克后4小时内坏死明显，注射低至5.0微克就能产生一个硬结区域（10×10毫米）。研究了来自六种细菌菌株的粘肽。对细胞壁和衍生粘肽的比较表明，随着残留多糖减少，急性坏死性病变往往更严重。用粘肽进行超免疫可减轻急性反应，有免疫特异性的证据。用溶菌酶孵育也会根据消化程度改变反应。毒性与颗粒大小有关，因为延长超声振动会降低活性。组织学切片显示多形核白细胞大量聚集，同时胶原发生改变。注射完整细胞壁片段约7天后出现慢性结节性病变。与急性坏死反应不同，这种病变很少由从多糖中分离出的粘肽产生。
