Villani L, Ciani F, Contestabile A
Anat Embryol (Berl). 1977 Dec 28;152(1):29-41. doi: 10.1007/BF00341433.
The ultrastructural localization of acetylcholinesterase (AChE) was studied in the cerebellar cortex of the quail by means of histochemical method. The greater amount of AChE was detected at leve of the molecular layer in the intracellular spaces between parallel fibers and between parallel fibers and dendritic terminals. Many neurons showed intracellular localization of enzyme activity: the AChE positive neurons were all Golgi cells, most stellate the basket cells and different aliquots of Purkinje and granule cells. The enzymatic activity was usually localized in the cisternae of endoplasmic reticulum, in the nuclear envelope (but this last localization was not present in Purkinje cells- and sometimes in the Golgi apparatus; reaction granules were usually scarce in the different dendritic branches ramifying in the molecular layer. On the basis of the ultrastructural pattern of AChE distribution, some considerations are developed on the methodological aspects concerning the reliability of histochemical methods, the differences recorded at light and electron microscope level, the problems related to extracellular localization of enzyme, the difficulty of establishing a precise correlation between AChE localization in a cerebellar neuron and its possible cholinergic and/or cholinoceptive nature.
采用组织化学方法研究了鹌鹑小脑皮质中乙酰胆碱酯酶（AChE）的超微结构定位。在分子层水平的平行纤维之间以及平行纤维与树突末端之间的细胞内空间中检测到了大量的AChE。许多神经元显示出酶活性的细胞内定位：AChE阳性神经元均为高尔基细胞，大多数为星状细胞、篮状细胞以及不同比例的浦肯野细胞和颗粒细胞。酶活性通常定位于内质网的池、核膜（但最后这种定位在浦肯野细胞中不存在，有时在高尔基器中也不存在）；在分子层中分支的不同树突分支中，反应颗粒通常很少。基于AChE分布的超微结构模式，对组织化学方法的可靠性、光镜和电镜水平记录的差异、与酶细胞外定位相关的问题、在小脑神经元中建立AChE定位与其可能的胆碱能和/或胆碱感受性性质之间精确相关性的困难等方法学方面进行了一些思考。
