作为对去甲肾上腺素的反应，心肌核糖核酸代谢主要方面的改变。利血平处理后该激素对核糖核酸胞质加工的作用。
Modifications of major aspects of myocardial ribonucleic acid metabolism as a response to noradrenaline. Action of the hormone on cytoplasmic processing of ribonucleic acid after reserpine treatment.
作者信息
Corti A, Casti A, Mezzetti G, Reali N, Orlandini G, Caldarera C M
出版信息
Biochem J. 1977 Dec 15;168(3):341-5. doi: 10.1042/bj1680341.
Treatment of perfused rabbit heart with reserpine causes a decrease of incorporation of labelled precursors into RNA species of subcellular fractions and polyamines. Ornithine decarboxylase, S-adenosylmethionine decarboxylase and cytoplasmic Mn2+-stimulated polyadenylate polymerase activities are not modified. Addition of noradrenaline to reserpine-treated perfused hearts enhances, compared with the control, the incorporation of precursor into RNA in all subcellular fractions other than the nuclear one, restores incorporation of labelled putrescine into polyamines, enhances ornithine decarboxylase and S-adenosylmethionine decarboxylase activities and causes a 12-fold increase in cytoplasmic Mn2+-dependent polyadenylate polymerase activity. After treatment with noradrenaline the increase in radioactivity was found solely in AMP after hydrolysis of microsomal RNA to nucleoside monophosphates.
用利血平处理灌注兔心脏会导致标记前体掺入亚细胞组分和多胺的RNA种类减少。鸟氨酸脱羧酶、S-腺苷甲硫氨酸脱羧酶和细胞质中受锰离子刺激的多腺苷酸聚合酶活性未发生改变。与对照相比，向用利血平处理的灌注心脏中添加去甲肾上腺素可增强除细胞核外所有亚细胞组分中前体掺入RNA的能力，恢复标记腐胺掺入多胺的能力，增强鸟氨酸脱羧酶和S-腺苷甲硫氨酸脱羧酶的活性，并使细胞质中依赖锰离子的多腺苷酸聚合酶活性增加12倍。用去甲肾上腺素处理后，微粒体RNA水解为核苷单磷酸后，放射性增加仅出现在AMP中。
Zotero 插件