Yashphe D J, Weiss D W
Clin Exp Immunol. 1970 Aug;7(2):269-81.
Studies were continued on the mode of action of a methanol-insoluble moiety (methanol-extraction residue) of attenuated tubercle bacilli which enhances the immune response. We analysed the effects of treatment with this material on the primary and secondary immune responses of mice to two antigens, sheep red blood cells and T phage. Pretreatment with methanol-extraction residue enhanced the primary response to sheep red blood cells, accelerating the formation of both 19S and 7S plaqueforming cells and of circulating haemagglutinins, and prolonging high levels of 19S and 7S plaque-forming cells. Methanol-extraction residue delayed the secondary response to sheep red blood cells when administered between primary and secondary immunization. Methanolextraction residue administration subsequent to immunization did not affect an ongoing primary response to this antigen. Administration of methanol-extraction residue before primary immunization with T phage, or between primary and secondary immunizations, enhanced both the primary and secondary circulating antibody responses to this antigen. The findings are discussed in relation to possible sites of action of methanol-extraction residue.
针对减毒结核杆菌的甲醇不溶性部分（甲醇提取残渣）增强免疫反应的作用方式展开了进一步研究。我们分析了用该物质处理对小鼠针对两种抗原（绵羊红细胞和T噬菌体）的初次和二次免疫反应的影响。用甲醇提取残渣进行预处理增强了对绵羊红细胞的初次反应，加速了19S和7S噬斑形成细胞以及循环血凝素的形成，并延长了19S和7S噬斑形成细胞的高水平状态。当在初次免疫和二次免疫之间给予甲醇提取残渣时，其延迟了对绵羊红细胞的二次反应。在免疫后给予甲醇提取残渣并不影响对该抗原正在进行的初次反应。在用T噬菌体进行初次免疫之前或在初次免疫和二次免疫之间给予甲醇提取残渣，增强了对该抗原的初次和二次循环抗体反应。结合甲醇提取残渣可能的作用位点对这些发现进行了讨论。
