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温度骤变对嗜温酵母和嗜冷酵母生长的影响。

Effect of abrupt temperature shift on the growth of mesophilic and psychrophilic yeasts.

作者信息

Shaw M K

出版信息

J Bacteriol. 1967 Apr;93(4):1332-6. doi: 10.1128/jb.93.4.1332-1336.1967.

DOI:10.1128/jb.93.4.1332-1336.1967
PMID:6067873
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC276605/
Abstract

Exponentially growing cultures of mesophilic and psychrophilic yeasts were subjected to abrupt changes in temperature. Temperature shifts made within the range in which the temperature characteristic, mu, is relatively constant (moderate temperatures) immediately induced growth at the normal exponential rate for the new temperature. Prior incubation at temperatures defined as moderate enabled some yeasts to grow for a few generations at temperatures higher than their normal maximal temperature for growth. Shifts made to or from temperatures above or below those in the moderate temperature range resulted in growth rates that were intermediate between the normal steady-state rates for the initial and final temperatures. A period of transient growth rate at the new temperature outside the moderate temperature range seems to be required before normal steady-state growth rates can be attained after such temperature shifts. The psychrophiles gave transient growth rates only below 10 C, whereas the mesophiles gave transient rates below 20 C. However, the psychrophiles cannot be distinguished from the mesophiles on the basis of the temperature characteristic, mu, which was found to be about 12,000 cal/mole for both types.

摘要

对嗜温酵母和嗜冷酵母的指数生长培养物进行温度的突然变化处理。在温度特征参数μ相对恒定的范围内(适度温度)进行的温度转换,会立即诱导其以新温度下的正常指数速率生长。在定义为适度的温度下预先培养,能使一些酵母在高于其正常生长最高温度的条件下生长几代。向适度温度范围之上或之下的温度进行转换,会导致生长速率介于初始温度和最终温度的正常稳态速率之间。在这种温度转换后,似乎需要在适度温度范围之外的新温度下经历一段短暂的生长速率期,才能达到正常的稳态生长速率。嗜冷菌仅在10℃以下出现短暂生长速率,而嗜温菌在20℃以下出现短暂生长速率。然而,根据温度特征参数μ无法区分嗜冷菌和嗜温菌,发现这两种类型的μ约为12,000卡/摩尔。

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本文引用的文献

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2
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Studies on the biochemical basis of the low maximum temperature in a psychrophilic cryptococcus.嗜冷隐球菌中低温最高温度的生化基础研究。
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Growth of psychrophilic bacteria.嗜冷细菌的生长
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Cold-sensitive mutants of Escherichia coli resulting from increased feedback inhibition.由增强的反馈抑制作用导致的大肠杆菌冷敏感突变体。
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