Pstrpw J D
J Clin Invest. 1967 Dec;46(12):2035-52. doi: 10.1172/JCI105692.
A technique is described for preparation in the guinea pig of an in situ, isolated, vascularized gall bladder that exhibits normal absorptive functions. Absorption of labeled bile pigments from the gall bladder was determined by the subsequent excretion of radioactivity in hepatic bile. Over a wide range of concentrations, unconjugated bilirubin-(14)C was well absorbed, whereas transfer of conjugated bilirubin proceeded slowly. Mesobilirubinogen-(3)H was absorbed poorly from whole bile, but was absorbed as rapidly as unconjugated bilirubin from a solution of pure conjugated bile salt. Bilirubin absorption was not impaired by iodoacetamide, 1.5 mM, or dinitrophenol, 1.0 mM, even though water transport was affected. This indicated that absorption of bilirubin was not dependent upon water transport, nor upon energy-dependent processes. The linear relationship between absorption and concentration of pigment at low concentrations in bile salt solutions suggested that pigment was transferred by passive diffusion. At higher pigment concentrations or in whole bile, this simple relationship was modified by interactions of pigment with bile salts and other constituents of bile. These interactions did not necessarily involve binding of bilirubin in micelles. The slow absorption of the more polar conjugates and photo-oxidative derivatives of bilirubin suggested that bilirubin was absorbed principally by nonionic, and partially, by ionic diffusion. Concentrations of pure conjugated bile salts above 3.5 mM were found to be injurious to the gall bladder mucosa. This mucosal injury did not affect the kinetics of bilirubin absorption. During in vitro incubation of bile at 37 degrees C, decay of bilirubin and hydrolysis of the conjugate proceeded as first-order reactions. The effects of these processes on the kinetics of bilirubin absorption, and their possible role in the formation of "white bile" and in the demonstrated appearance of unconjugated bilirubin in hepatic bile, are discussed.
本文描述了一种在豚鼠体内制备原位、分离、血管化且具有正常吸收功能的胆囊的技术。通过随后肝胆汁中放射性物质的排泄来测定胆囊对标记胆汁色素的吸收。在很宽的浓度范围内，未结合胆红素 -（14）C 被很好地吸收，而结合胆红素的转运则很缓慢。中胆素原 -（3）H 从全胆汁中吸收较差，但从纯结合胆汁盐溶液中吸收的速度与未结合胆红素一样快。即使水转运受到影响，1.5 mM 的碘乙酰胺或 1.0 mM 的二硝基苯酚也不会损害胆红素的吸收。这表明胆红素的吸收不依赖于水转运，也不依赖于能量依赖过程。在胆盐溶液中低浓度时，色素吸收与浓度之间的线性关系表明色素是通过被动扩散转运的。在较高色素浓度或全胆汁中，这种简单关系因色素与胆盐及胆汁其他成分的相互作用而改变。这些相互作用不一定涉及胆红素在微胶粒中的结合。胆红素极性更强的结合物和光氧化衍生物吸收缓慢，这表明胆红素主要通过非离子扩散吸收，部分通过离子扩散吸收。发现纯结合胆汁盐浓度高于 3.5 mM 时对胆囊黏膜有损害。这种黏膜损伤不影响胆红素吸收的动力学。在 37℃体外孵育胆汁期间，胆红素的衰变和结合物的水解以一级反应进行。讨论了这些过程对胆红素吸收动力学的影响，以及它们在“白胆汁”形成和肝胆汁中未结合胆红素出现中可能起的作用。
