RNA稳定性的热力学研究。
Thermodynamic studies of RNA stability.
作者信息
Freier S M, Petersheim M, Hickey D R, Turner D H
机构信息
Department of Chemistry, University of Rochester, New York 14627.
出版信息
J Biomol Struct Dyn. 1984 Mar;1(5):1229-42. doi: 10.1080/07391102.1984.10507514.
Enthalpies and entropies of helix stabilization due to addition of 3' terminal unpaired nucleotides to a CCGG or GGCC core double helix are derived from UV melting studies. The results suggest stacking provides a significant fraction of the free energy of a terminal base pair. The effects of temperature, aggregation, and ionic strength on the determination of thermodynamic parameters are considered. Helix propagation parameters are revised and extended based on recent additions to the data set.
通过紫外熔解研究得出了向CCGG或GGCC核心双螺旋添加3'末端未配对核苷酸所导致的螺旋稳定化的焓和熵。结果表明，堆积作用提供了末端碱基对自由能的很大一部分。考虑了温度、聚集和离子强度对热力学参数测定的影响。基于数据集的最新补充，对螺旋延伸参数进行了修订和扩展。
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