基因组DNA的印迹杂交分析
Blot hybridisation analysis of genomic DNA.
作者信息
Vandenplas S, Wiid I, Grobler-Rabie A, Brebner K, Ricketts M, Wållis G, Bester A, Boyd C, Måthew C
出版信息
J Med Genet. 1984 Jun;21(3):164-72. doi: 10.1136/jmg.21.3.164.
Abstract
Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.
摘要
特定基因序列的限制性内切酶分析已被证明是一种用于遗传性疾病特征鉴定和诊断的有价值技术。本文描述了基因组DNA分离、酶切和印迹杂交的详细方案。讨论了该过程中的问题及替代方法，并提供了故障排除指南以帮助纠正错误。
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