Bale J F, O'Neil M E, Hogan R N, Kern E R
Arch Ophthalmol. 1984 Aug;102(8):1214-9. doi: 10.1001/archopht.1984.01040030984032.
To study experimental cytomegalovirus (CMV) infection of ocular structures, 3-week-old Swiss-Webster mice were inoculated intraperitoneally with a 0% to 20% lethal inoculum of murine CMV (MCMV). Murine cytomegalovirus was recovered from homogenates of eye tissues on days 3, 5, and 7 after inoculation. Peak virus titers, mean of 2.93 +/- 0.67 log plaque-forming units of MCMV per gram of ocular tissue occurred on day 5. Urine cytomegalovirus was recovered from explant cultures of eye and optic nerve 14, 21, 60, 90, and 120 days after MCMV inoculation. Murine cytomegalovirus also persisted in intraocular fluids for as long as 90 days. Murine cytomegalovirus infection was confirmed by immunofluorescence antibody staining and transmission electron microscopy. These experiments indicate that MCMV infects the ocular tissues of mice during systemic MCMV infection, and persists in ocular tissues for as long as 120 days after infection. These studies may be directly relevant to ocular disorders that occur during acquired CMV infections of humans, and suggest that ocular tissues may be a site of CMV persistence or latency.
为研究眼部结构的实验性巨细胞病毒（CMV）感染，给3周龄的瑞士韦伯斯特小鼠腹腔注射0%至20%致死剂量的鼠巨细胞病毒（MCMV）。接种后第3、5和7天从眼组织匀浆中分离出鼠巨细胞病毒。病毒滴度峰值在第5天出现，每克眼组织平均为2.93±0.67 log空斑形成单位的MCMV。接种MCMV后14、21、60、90和120天，从眼和视神经的外植体培养物中分离出尿巨细胞病毒。鼠巨细胞病毒也在眼内液中持续存在长达90天。通过免疫荧光抗体染色和透射电子显微镜确认了鼠巨细胞病毒感染。这些实验表明，在全身性MCMV感染期间，MCMV感染小鼠的眼组织，并在感染后长达120天内在眼组织中持续存在。这些研究可能与人类获得性CMV感染期间发生的眼部疾病直接相关，并表明眼组织可能是CMV持续存在或潜伏的部位。
