Kilpatrick D C, Graham C, Urbaniak S J, Jeffree C E, Allen A K
Biochem J. 1984 Jun 15;220(3):843-7. doi: 10.1042/bj2200843.
A deglycosylated derivative of tomato (Lycopersicon esculentum) lectin was prepared with the use of trifluoromethanesulphonic acid. Its properties were generally similar to those of the native lectin, but differences were evident in terms of relative agglutinating activity towards sheep, (untreated) human and trypsin-treated human erythrocytes. The two forms of tomato lectin were used in conjunction with a battery of specific antisera to investigate structural relatedness among solanaceous lectins. Immunological cross-reactivity between tomato, potato and Datura lectins depends on the integrity of the glycosylated region of those lectins; that between Datura lectin and other seed lectins, however, has a separate structural basis.
利用三氟甲磺酸制备了番茄（番茄属）凝集素的去糖基化衍生物。其性质总体上与天然凝集素相似，但在对绵羊、（未处理的）人以及经胰蛋白酶处理的人红细胞的相对凝集活性方面存在明显差异。两种形式的番茄凝集素与一系列特异性抗血清结合使用，以研究茄科凝集素之间的结构相关性。番茄、马铃薯和曼陀罗凝集素之间的免疫交叉反应取决于这些凝集素糖基化区域的完整性；然而，曼陀罗凝集素与其他种子凝集素之间的免疫交叉反应有单独的结构基础。
