Schedl T, Owens J, Dove W F, Burland T G
Genetics. 1984 Sep;108(1):143-64. doi: 10.1093/genetics/108.1.143.
The organization of the alpha- and beta-tubulin gene families in Physarum was investigated by Mendelian analysis. Restriction endonuclease-generated DNA fragments homologous to alpha- and beta-tubulin show length polymorphisms that can be used as markers for genetic mapping. Analysis of meiotic assortment among progeny of heterozygotes allowed alpha- and beta-tubulin sequence loci to be defined. There are four unlinked alpha-tubulin sequence loci (altA, altB, altC and altD) and at least three unlinked beta-tubulin sequence loci (betA, betB and betC). The alpha-tubulin loci are not linked to the beta-tubulin loci. --Segregation of tubulin sequence loci with respect to ben mutations that confer resistance to antitubulin benzimidazole drugs was used to investigate whether any members of the alpha- or beta-tubulin gene families are allelic to ben loci. The beta-tubulin sequence locus betB is allelic to the resistance locus benD, the betA locus is probably allelic to benA and the alpha-tubulin sequence locus altC may be allelic to benC. The molecular implications of benzimidazole resistance phenotypes when only one of the expressed beta-tubulin gene family members mutates to drug resistance are discussed in relation to tubulin function.
通过孟德尔分析研究了绒泡菌中α-微管蛋白和β-微管蛋白基因家族的组织情况。与α-微管蛋白和β-微管蛋白同源的限制性内切酶产生的DNA片段显示出长度多态性，可作为遗传图谱标记。对杂合子后代减数分裂分离情况的分析确定了α-微管蛋白和β-微管蛋白序列位点。有四个不连锁的α-微管蛋白序列位点（altA、altB、altC和altD）和至少三个不连锁的β-微管蛋白序列位点（betA、betB和betC）。α-微管蛋白位点与β-微管蛋白位点不连锁。——利用微管蛋白序列位点相对于赋予抗微管蛋白苯并咪唑药物抗性的ben突变的分离情况，研究α-或β-微管蛋白基因家族的任何成员是否与ben位点等位。β-微管蛋白序列位点betB与抗性位点benD等位，betA位点可能与benA等位，α-微管蛋白序列位点altC可能与benC等位。结合微管蛋白功能，讨论了仅一个表达的β-微管蛋白基因家族成员突变为药物抗性时苯并咪唑抗性表型的分子意义。
