Maréchal L, Guillemaut P, Grienenberger J M, Jeannin G, Weil J H
Nucleic Acids Res. 1985 Jun 25;13(12):4411-6. doi: 10.1093/nar/13.12.4411.
Bean mitochondria and chloroplast tRNAsTrp, purified by RPC-5 chromatography and two-dimensional gel electrophoresis, have been sequenced using post-labeling techniques. The high degree of sequence homology between bean mitochondria and chloroplast tRNAsTrp shows that these two tRNAs are coded for by closely related genes which have probably evolved from a common ancestor gene. The anticodon of bean mitochondria tRNATrp is CmCA, which can recognize UGG (the codon for tryptophan in the universal code) and is complementary neither to UGA (which codes for tryptophan in mammalian and yeast mitochondria) nor to CGG (which could be a tryptophan codeword in plant mitochondria).
菜豆线粒体和叶绿体的色氨酸转运RNA(tRNAsTrp),经RPC - 5色谱法和二维凝胶电泳纯化后,采用后标记技术进行了测序。菜豆线粒体和叶绿体的色氨酸转运RNA之间高度的序列同源性表明,这两种转运RNA由密切相关的基因编码,这些基因可能是从一个共同的祖先基因进化而来的。菜豆线粒体色氨酸转运RNA的反密码子是CmCA,它能识别UGG(通用密码中色氨酸的密码子),既不与UGA(在哺乳动物和酵母线粒体中编码色氨酸)互补,也不与CGG(可能是植物线粒体中的色氨酸密码子)互补。