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人β-干扰素基因在小鼠细胞中的染色体外调控表达。

Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.

作者信息

Zinn K, Mellon P, Ptashne M, Maniatis T

出版信息

Proc Natl Acad Sci U S A. 1982 Aug;79(16):4897-901. doi: 10.1073/pnas.79.16.4897.

DOI:10.1073/pnas.79.16.4897
PMID:6956899
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346792/
Abstract

Beta(fibroblast)-interferon mRNA and protein are induced by the synthetic double-stranded RNA poly(I) X poly(C) in cultured human fibroblasts. To study the mechanism of this induction, we have isolated a human beta-interferon gene and inserted it in a vector plasmid containing DNA of the bovine papilloma virus. After removal of bacterial plasmid sequences, the bovine papilloma virus-beta-interferon recombinant was used to morphologically transform mouse fibroblasts. Analysis of DNA from the transformed cell lines indicated that this recombinant is propagated as a stable multicopy extrachromosomal element. Human beta-interferon mRNA and protein are inducible by poly(I) X poly(C) in all of these cell lines, and the mRNA is indistinguishable from beta-interferon mRNA synthesized by induced human cells.

摘要

β（成纤维细胞）-干扰素mRNA和蛋白质在培养的人成纤维细胞中由合成的双链RNA聚肌苷酸×聚胞苷酸诱导产生。为了研究这种诱导的机制，我们分离了一个人β-干扰素基因，并将其插入到含有牛乳头瘤病毒DNA的载体质粒中。去除细菌质粒序列后，牛乳头瘤病毒-β-干扰素重组体被用于对小鼠成纤维细胞进行形态转化。对转化细胞系的DNA分析表明，这种重组体作为一种稳定的多拷贝染色体外元件进行增殖。在所有这些细胞系中，人β-干扰素mRNA和蛋白质都可被聚肌苷酸×聚胞苷酸诱导，并且该mRNA与诱导的人细胞合成的β-干扰素mRNA没有区别。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/50cb93afcd01/pnas00455-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/65f9d02a7827/pnas00455-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/d634aec01e76/pnas00455-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/50cb93afcd01/pnas00455-0074-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/65f9d02a7827/pnas00455-0073-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/d634aec01e76/pnas00455-0074-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/081d/346792/50cb93afcd01/pnas00455-0074-b.jpg

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Regulated expression of an extrachromosomal human beta-interferon gene in mouse cells.人β-干扰素基因在小鼠细胞中的染色体外调控表达。
Proc Natl Acad Sci U S A. 1982 Aug;79(16):4897-901. doi: 10.1073/pnas.79.16.4897.
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Sequences involved in the regulated expression of the human interferon-beta1 gene in recombinant SV40 DNA vectors replicating in monkey cells.在猴细胞中复制的重组SV40 DNA载体中，参与人β-干扰素1基因调控表达的序列。
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A bovine papilloma virus vector with a dominant resistance marker replicates extrachromosomally in mouse and E. coli cells.带有显性抗性标记的牛乳头瘤病毒载体在小鼠和大肠杆菌细胞中进行染色体外复制。
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3
Human fibroblast interferon RNA transcripts of different sizes in poly(I).poly(C) induced cells.

本文引用的文献

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Structure of a chromosomal gene for human interferon beta.人干扰素β基因的结构。
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5305-9. doi: 10.1073/pnas.78.9.5305.
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Bovine papillomavirus vector that propagates as a plasmid in both mouse and bacterial cells.牛乳头瘤病毒载体，它在小鼠和细菌细胞中均以质粒形式繁殖。
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Production of human beta interferon in insect cells infected with a baculovirus expression vector.在感染杆状病毒表达载体的昆虫细胞中生产人β干扰素。
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The 5'-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription.人类β-干扰素1基因的5'侧翼序列负责病毒诱导的转录。
Nucleic Acids Res. 1983 Aug 25;11(16):5403-12. doi: 10.1093/nar/11.16.5403.
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Expression and regulation of a human metallothionein gene carried on an autonomously replicating shuttle vector.一个携带在自主复制穿梭载体上的人类金属硫蛋白基因的表达与调控
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Variable-sized free episomes of Shope papilloma virus DNA are present in all non-virus-producing neoplasms and integrated episomes are detected in some.肖普乳头瘤病毒DNA大小可变的游离附加体存在于所有不产生病毒的肿瘤中，并且在一些肿瘤中检测到整合的附加体。
Proc Natl Acad Sci U S A. 1982 Feb;79(3):790-4. doi: 10.1073/pnas.79.3.790.
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Arrest of segregation leads to accumulation of highly intertwined catenated dimers: dissection of the final stages of SV40 DNA replication.分离停滞导致高度缠结的连环二聚体积累：SV40 DNA复制最后阶段的剖析
Cell. 1981 Sep;25(3):659-69. doi: 10.1016/0092-8674(81)90173-2.
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Mouse cells transformed by bovine papillomavirus contain only extrachromosomal viral DNA sequences.被牛乳头瘤病毒转化的小鼠细胞仅含有染色体外的病毒DNA序列。
Proc Natl Acad Sci U S A. 1981 May;78(5):2727-31. doi: 10.1073/pnas.78.5.2727.
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In vitro tumorigenic transformation by a defined sub-genomic fragment of bovine papilloma virus DNA.牛乳头瘤病毒DNA特定亚基因组片段的体外致瘤转化
Nature. 1980 Sep 4;287(5777):72-4. doi: 10.1038/287072a0.
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Controlled transcription of a human alpha-interferon gene introduced into mouse L cells.导入小鼠L细胞的人α-干扰素基因的可控转录。
Nature. 1982 May 13;297(5862):128-32. doi: 10.1038/297128a0.
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Analysis of interferon mRNA in human fibroblast cells induced to produce interferon.对诱导产生干扰素的人成纤维细胞中干扰素信使核糖核酸的分析。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7426-30. doi: 10.1073/pnas.78.12.7426.
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Inducer-responsive expression of the cloned human interferon beta 1 gene introduced into cultured mouse cells.导入培养的小鼠细胞中的克隆人干扰素β1基因的诱导物应答性表达。
Nucleic Acids Res. 1982 Feb 11;10(3):967-77. doi: 10.1093/nar/10.3.967.