冈田酸对正常（KMS-6）、永生化（KMST-6）和肿瘤转化（KMST-6T和KMST-6/RAS）人成纤维细胞的细胞生长、不依赖贴壁生长及共培养的影响。
Effects of okadaic acid on cell growth, anchorage-independent growth, and co-cultures of normal (KMS-6), immortalized (KMST-6), and neoplastically transformed (KMST-6T and KMST-6/RAS) human fibroblasts.
作者信息
Jahan I, Iijima M, Kondo T, Namba M
机构信息
Department of Cell Biology, Institute of Cellular and Molecular Biology, Okayama Univeristy Medical School, Japan.
出版信息
J Cancer Res Clin Oncol. 1996;122(4):249-53. doi: 10.1007/BF01209654.
The effects of okadaic acid (OA) on normal human (KMS-6), its immortalized (KMST-6) and neoplastically transformed (KMST-6T and KMST-6/RAS) cells were investigated as a model of two stage carcinogenesis. The presence of OA inhibited cell growth of the normal and immortalized cells but not that of the neoplastic KMST-6T cells. In contrast, cell growth of the other neoplastic KMST-6/RAS cells transformed with the Ha-ras oncogene was inhibited by OA. OA enhanced colony formation of KMST-6T cells in soft agar, but it suppressed that of KMST-6/RAS cells. Co-cultures of KMST-6T cells with normal KMS-6 cells showed an increase in focus formation of KMST-6T cells in the presence of OA, whereas focus formation of KMST-6/RAS cells decreased. These results indicate that OA has growth-promoting effects on certain types of transformed human cells.
作为两阶段致癌模型，研究了冈田酸（OA）对正常人细胞（KMS - 6）、其永生化细胞（KMST - 6）和肿瘤转化细胞（KMST - 6T和KMST - 6/RAS）的影响。OA的存在抑制了正常细胞和永生化细胞的生长，但对肿瘤性KMST - 6T细胞的生长没有抑制作用。相反，用Ha - ras癌基因转化的另一种肿瘤性KMST - 6/RAS细胞的生长受到OA的抑制。OA增强了KMST - 6T细胞在软琼脂中的集落形成，但抑制了KMST - 6/RAS细胞的集落形成。在OA存在的情况下，KMST - 6T细胞与正常KMS - 6细胞的共培养显示KMST - 6T细胞的灶形成增加，而KMST - 6/RAS细胞的灶形成减少。这些结果表明，OA对某些类型的转化人细胞具有促生长作用。
Zotero 插件