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一种用于测定多形核白细胞产生氧化剂的定量荧光测定法：其在细胞活化过程同步荧光测定中的应用。

A quantitative fluorimetric assay for the determination of oxidant production by polymorphonuclear leukocytes: its use in the simultaneous fluorimetric assay of cellular activation processes.

作者信息

Hyslop P A, Sklar L A

出版信息

Anal Biochem. 1984 Aug 15;141(1):280-6. doi: 10.1016/0003-2697(84)90457-3.

DOI:10.1016/0003-2697(84)90457-3
PMID:6093625
Abstract

A fluorimetric assay for the indirect determination of superoxide production during the respiratory burst of stimulated polymorphonuclear leukocytes was described. The method allowed the determination of submicromolar concentrations of superoxide, and was sufficiently sensitive that first-derivative kinetic analysis of the respiratory burst could be quickly analyzed. Conditions for the simultaneous fluorimetric analysis of superoxide production and intracellular calcium fluxes were described.

摘要

描述了一种用于间接测定刺激的多形核白细胞呼吸爆发期间超氧化物产生的荧光测定法。该方法能够测定亚微摩尔浓度的超氧化物，灵敏度足以快速分析呼吸爆发的一阶动力学分析。描述了同时荧光分析超氧化物产生和细胞内钙通量的条件。

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A quantitative fluorimetric assay for the determination of oxidant production by polymorphonuclear leukocytes: its use in the simultaneous fluorimetric assay of cellular activation processes.一种用于测定多形核白细胞产生氧化剂的定量荧光测定法：其在细胞活化过程同步荧光测定中的应用。
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