与ptsM基因座距离较远的基因座，影响大肠杆菌K-12中酶IIA/IIB的功能。
Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12.
作者信息
Roehl R A, Vinopal R T
出版信息
J Bacteriol. 1980 Apr;142(1):120-30. doi: 10.1128/jb.142.1.120-130.1980.
Abstract
Most strains of Escherichia coli K-12 are unable to use the enzyme IIA/IIB (enzyme IIMan) complex of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in anaerobic growth and therefore cannot utilize glucosamine anaerobically. Introduction into these strains of a ptsG mutation, which eliminates activity of the enzyme IIIGlc/IIB' complex of the PTS, resulted in inability to grow anaerobically on glucose and mannose. Derivative strains able to grow anaerobically on glucosamine had mutations at a locus close to man, the gene coding for phosphomannose isomerase, and had higher enzyme IIA/IIB activities during anaerobic growth than did the parental strain. These results establish a locus affecting function of enzyme IIA/IIB that maps distant from ptsM, the probable structural gene for enzyme IIB.
摘要
大多数大肠杆菌K-12菌株在厌氧生长时无法利用磷酸烯醇丙酮酸：糖磷酸转移酶系统（PTS）的酶IIA/IIB（酶IIMan）复合物，因此不能厌氧利用氨基葡萄糖。将ptsG突变引入这些菌株，该突变消除了PTS的酶IIIGlc/IIB'复合物的活性，导致无法在葡萄糖和甘露糖上厌氧生长。能够在氨基葡萄糖上厌氧生长的衍生菌株在靠近man的位点发生了突变，man是编码磷酸甘露糖异构酶的基因，并且在厌氧生长期间比亲本菌株具有更高的酶IIA/IIB活性。这些结果确定了一个影响酶IIA/IIB功能的位点，该位点与ptsM（酶IIB的可能结构基因）相距较远。
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