Ivannikova T A, Korolev M B, Shekoian L A
Vopr Virusol. 1984 Sep-Oct;29(5):589-92.
The mechanism of rotavirus antigen amplification in cell culture was studied by immunofluorescence. Reproduction of human rotavirus in cell culture was shown to begin 6 h after inoculation and to be accompanied by a regular increase in the number of antigen-containing cells. A good correlation with the results of electron microscopic studies was demonstrated. The possibility of reliable detection of rotavirus antigen in cell culture by the fluorescent antibody technique at relatively early intervals after infection has been established.
通过免疫荧光法研究了轮状病毒抗原在细胞培养中的扩增机制。结果表明，人轮状病毒在细胞培养中的复制在接种后6小时开始，并伴随着含抗原细胞数量的规律性增加。结果显示与电子显微镜研究结果具有良好的相关性。已证实采用荧光抗体技术在感染后相对早期阶段能够可靠地检测细胞培养中的轮状病毒抗原。
