[Immunofluorescent study of the reproduction of human rotavirus in cell culture].

The mechanism of rotavirus antigen amplification in cell culture was studied by immunofluorescence. Reproduction of human rotavirus in cell culture was shown to begin 6 h after inoculation and to be accompanied by a regular increase in the number of antigen-containing cells. A good correlation with the results of electron microscopic studies was demonstrated. The possibility of reliable detection of rotavirus antigen in cell culture by the fluorescent antibody technique at relatively early intervals after infection has been established.