BinI：一种来自婴儿双歧杆菌的新型位点特异性核酸内切酶。

BinI: a new site-specific endonuclease from Bifidobacterium infantis.

作者信息

Khosaka T, Kiwaki M

出版信息

Gene. 1984 Nov;31(1-3):251-5. doi: 10.1016/0378-1119(84)90217-8.

Abstract

A new restriction endonuclease, BinI, from Bifidobacterium infantis 659 has been isolated. By mapping and sequencing of the cleavage sites on SV40 DNA, it was deduced that BinI recognizes the asymmetric pentanucleotide sequence (formula; see text) and cleaves at the sites indicated by the arrows, generating mononucleotide 5'-terminal extensions. BinI is a member of a unique class of Type-II restriction endonucleases which recognize a specific but asymmetric sequence and cleave at a site several bases away.

摘要

已从婴儿双歧杆菌659中分离出一种新的限制性内切酶BinI。通过对SV40 DNA上切割位点的图谱绘制和测序，推断出BinI识别不对称五核苷酸序列（分子式；见正文）并在箭头所示位点切割，产生单核苷酸5'-末端延伸。BinI是一类独特的II型限制性内切酶的成员，这类酶识别特定但不对称的序列并在几个碱基外的位点切割。