来自婴儿双歧杆菌的两种位点特异性内切酶BinSI和BinSII。
Two site-specific endonucleases BinSI and BinSII from Bifidobacterium infantis.
作者信息
Khosaka T, Kiwaki M, Rak B
出版信息
FEBS Lett. 1983 Nov 14;163(2):170-4. doi: 10.1016/0014-5793(83)80812-6.
PMID:6315484
Abstract
Two site-specific endonucleases, BinSI and BinSII, were isolated from Bifidobacterium infantis S76e. BinSI was found to be an isoschizomer of EcoRII, while BinSII was shown to have the same sequence and cutting specificity as BbeI, 5'-GGCGC decreases C-3'. Both BinSII- and BbeI-generated DNA fragments could be ligated with HaeII-generated DNA fragments.
摘要
从婴儿双歧杆菌S76e中分离出两种位点特异性内切酶BinSI和BinSII。发现BinSI是EcoRII的同裂酶，而BinSII显示出与BbeI相同的序列和切割特异性，即5'-GGCGC↓C-3'。BinSII和BbeI产生的DNA片段都可以与HaeII产生的DNA片段连接。
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