Makula R A, Meagher R B
Nucleic Acids Res. 1980 Jul 25;8(14):3125-31. doi: 10.1093/nar/8.14.3125.
The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping an ligation.
报道了从挪威脱硫弧菌(Desulfovibrio desulfuricans)这种硫酸盐还原厌氧菌中分离出一种新的限制性内切酶Dde 1,并对其进行了纯化和特性鉴定。该酶识别序列(见公式索引)并在箭头所示位置切割。所获得的酶制剂适用于限制性图谱绘制和连接。