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一种来自挪威脱硫弧菌(Desulfovibrio desulfuricans)这种厌氧菌的新型限制性内切酶。

A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway.

作者信息

Makula R A, Meagher R B

出版信息

Nucleic Acids Res. 1980 Jul 25;8(14):3125-31. doi: 10.1093/nar/8.14.3125.

DOI:10.1093/nar/8.14.3125
PMID:6255409
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324359/
Abstract

The purification and characterization of a new restriction endonuclease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence (see formula index) and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping an ligation.

摘要

报道了从挪威脱硫弧菌(Desulfovibrio desulfuricans)这种硫酸盐还原厌氧菌中分离出一种新的限制性内切酶Dde 1,并对其进行了纯化和特性鉴定。该酶识别序列(见公式索引)并在箭头所示位置切割。所获得的酶制剂适用于限制性图谱绘制和连接。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/beeb34b36b5d/nar00431-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/94635579d287/nar00431-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/5905da0fd094/nar00431-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/beeb34b36b5d/nar00431-0070-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/94635579d287/nar00431-0068-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/5905da0fd094/nar00431-0069-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c868/324359/beeb34b36b5d/nar00431-0070-a.jpg

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Characterization of a small plasmid from Desulfovibrio desulfuricans and its use for shuttle vector construction.脱硫脱硫弧菌小质粒的特性及其在穿梭载体构建中的应用。
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本文引用的文献

1
A computer assisted method for the determination of restriction enzyme recognifion sites.一种用于确定限制性内切酶识别位点的计算机辅助方法。
Nucleic Acids Res. 1978 Nov;5(11):4105-27. doi: 10.1093/nar/5.11.4105.
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A specific endonuclease from Bacillus caldolyticus.一种来自嗜热解芽孢杆菌的特异性核酸内切酶。
Nucleic Acids Res. 1978 Oct;5(10):3457-67. doi: 10.1093/nar/5.10.3457.
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A general method for the purification of restriction enzymes.一种纯化限制性内切酶的通用方法。
Restriction and modification enzymes and their recognition sequences.
限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1982 Mar 11;10(5):r117-44. doi: 10.1093/nar/10.5.1770.
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Direct identification of sickle cell anemia by blot hybridization.通过印迹杂交直接鉴定镰状细胞贫血。
Proc Natl Acad Sci U S A. 1981 Aug;78(8):5081-5. doi: 10.1073/pnas.78.8.5081.
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Characterization of a site-specific restriction endonuclease from Streptomyces aureofaciens.来自金色链霉菌的位点特异性限制性内切酶的特性分析。
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Cloning the DdeI restriction-modification system using a two-step method.采用两步法克隆DdeI限制修饰系统。
Nucleic Acids Res. 1986 Oct 24;14(20):7939-51. doi: 10.1093/nar/14.20.7939.
7
Restriction and modification enzymes and their recognition sequences.限制酶和修饰酶及其识别序列。
Nucleic Acids Res. 1985;13 Suppl(Suppl):r165-200. doi: 10.1093/nar/13.suppl.r165.
8
Restriction enzymes and their isoschizomers.限制性内切酶及其同裂酶。
Nucleic Acids Res. 1988;16 Suppl(Suppl):r271-313. doi: 10.1093/nar/16.suppl.r271.
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Restriction enzymes and their isoschizomers.限制性内切酶及其同裂酶。
Nucleic Acids Res. 1989;17 Suppl(Suppl):r347-87. doi: 10.1093/nar/17.suppl.r347.
10
Restriction enzymes and their isoschizomers.限制性内切酶及其同裂酶。
Nucleic Acids Res. 1990 Apr 25;18 Suppl(Suppl):2331-65. doi: 10.1093/nar/18.suppl.2331.
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A restriction enzyme from Fusobacterium nucleatum 4H which recognizes GCNGC.一种来自具核梭杆菌4H的限制性内切酶,其识别GCNGC。
Nucleic Acids Res. 1979 Jan;6(1):17-25. doi: 10.1093/nar/6.1.17.
5
Site specific endonuclease from Fusobacterium nucleatum.具核梭杆菌的位点特异性内切酶。
Nucleic Acids Res. 1979 Jan;6(1):1-15. doi: 10.1093/nar/6.1.1.
6
Specificity of substrate recognition by the EcoRI restriction endonuclease.EcoRI限制性内切核酸酶对底物识别的特异性
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4. doi: 10.1073/pnas.72.9.3310.