Sugisaki H, Kanazawa S
Gene. 1981 Dec;16(1-3):73-8. doi: 10.1016/0378-1119(81)90062-7.
Two new restriction endonucleases have been isolated from Flavobacterium okeanokoites IFO12536 and Micrococcus luteus IFO12992 and named FokI and MluI, respectively. Based on analysis of the sequences around the restriction sites, the recognition sequences and cleavage sites of these endonucleases were deduced as below: FokI: (formula: see text). MluI introduces double-strand cleavages at unique sequences that are completely two-fold rotationally symmetric like most type II restriction endonucleases. FokI belongs to a class of restriction endonucleases that recognize specific but asymmetric nucleotide sequences and introduce staggered cleavages at appointed positions away from the recognition sequences.
已从海洋黄杆菌IFO12536和藤黄微球菌IFO12992中分离出两种新的限制性内切酶，分别命名为FokI和MluI。根据对限制位点周围序列的分析，推导出这些内切酶的识别序列和切割位点如下：FokI：（公式：见原文）。MluI像大多数II型限制性内切酶一样，在完全具有二重旋转对称性的独特序列处引入双链切割。FokI属于一类识别特定但不对称核苷酸序列并在远离识别序列的指定位置引入交错切割的限制性内切酶。
