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成年牛蛙(牛蛙属)大脑及其他器官中单胺氧化酶活性的特征

Characteristics of monoamine oxidase activity in brain and other organs of the adult bullfrog, Rana catesbeiana.

作者信息

Urueña G, Hall T R

出版信息

Comp Biochem Physiol C Comp Pharmacol. 1982;71C(1):95-100. doi: 10.1016/0306-4492(82)90016-8.

DOI:10.1016/0306-4492(82)90016-8
PMID:6121676
Abstract
  1. Monoamine oxidase (MAO) activity was measured in brain, liver, kidney and intestine of the adult bullfrog by a fluorometric method. 2. All tissues contained both type A and type B MAO, on the basis of responses to specific inhibitors, but with different ratios in each tissue. 3. MAO activity was optimum at 30 degrees C. However, MAO type B showed greater activity changes related to incubation temperature than did type A. 4. The Michaelis constant (Km) of MAO also varied with temperature, with a nadir around 20 degrees C. The functional significance of this is not clear. 5. Arrhenius plots showed that the activation energy for MAO B was higher than for MAO A.
摘要
  1. 采用荧光法测定成年牛蛙脑、肝、肾和肠道中的单胺氧化酶(MAO)活性。2. 根据对特异性抑制剂的反应,所有组织均含有A型和B型MAO,但各组织中二者比例不同。3. MAO活性在30℃时最佳。然而,B型MAO相较于A型MAO,其活性变化与孵育温度的相关性更大。4. MAO的米氏常数(Km)也随温度变化,在20℃左右出现最低点。其功能意义尚不清楚。5. 阿累尼乌斯图表明,B型MAO的活化能高于A型MAO。

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