Czirók E, Orskov I, Orskov F
Infect Immun. 1982 Aug;37(2):519-25. doi: 10.1128/iai.37.2.519-525.1982.
Bacterial agglutination and crossed immunoelectrophoresis were compared as techniques for the subdivision of mannose-resistant hemagglutinating Escherichia coli fimbrial antigens. A total of 22 fimbrial strains, 15 of which had earlier been grouped into two fimbrial agglutination groups, were examined for the presence of fimbrial antigens F7 go F12 by crossed immunoelectrophoresis. Most of the strains were isolated from infants with diarrhea; they were neither enteropathogenic nor enterotoxigenic serotypes. Of the 10 strains in agglutination group 1, 4 had fimbrial antigens F7 and 1C, 4 had antigens F8 and 1C, and in 1 strain, only antigen 1C was demonstrated. The tenth strain did not, by the crossed immunoelectrophoresis test, fit into agglutination group 1. Agglutination group 2 comprised five strains. Four of these had antigens in common with the still unnumbered fimbrial antigens of an O4:K12:H5 strain. In the fifth strain, no known F antigens were demonstrated. The previously found correlation between some O:K:H serotypes and fimbrial antigens in strains from urinary tract infections was confirmed in this study in strains from diarrhea cases. We concluded that, although the agglutination test can indicate the presence of certain fimbriae, it cannot be used presently for an exact demonstration of these antigens because each strain often produces several different fimbriae.
对细菌凝集试验和交叉免疫电泳进行了比较，以作为对甘露糖抗性血凝性大肠杆菌菌毛抗原进行细分的技术。总共检查了22株菌毛菌株，其中15株先前已被归入两个菌毛凝集组，通过交叉免疫电泳检测菌毛抗原F7至F12的存在情况。大多数菌株是从腹泻婴儿中分离出来的；它们既不是肠致病性血清型也不是产肠毒素血清型。在凝集组1的10株菌株中，4株有菌毛抗原F7和1C，4株有抗原F8和1C，1株仅显示出抗原1C。通过交叉免疫电泳试验，第十株菌株不属于凝集组1。凝集组2包括五株菌株。其中四株与一株O4:K12:H5菌株尚未编号的菌毛抗原有共同抗原。在第五株菌株中，未显示出已知的F抗原。本研究在腹泻病例菌株中证实了先前在尿路感染菌株中发现的某些O:K:H血清型与菌毛抗原之间的相关性。我们得出结论，虽然凝集试验可以表明某些菌毛的存在，但目前不能用于准确显示这些抗原，因为每个菌株通常会产生几种不同的菌毛。
