Esch F S, Böhlen P, Ling N C, Brazeau P E, Wehrenberg W B, Guillemin R
J Biol Chem. 1983 Feb 10;258(3):1806-12.
The primary structures of three polypeptides, possessing high intrinsic growth hormone-releasing activity and derived from a human pancreatic carcinoma which had caused acromegaly, were established by sequence analyses of the intact peptides and their cyanogen bromide digestion fragments with a gas-phase sequenator. The three human pancreas growth hormone-releasing factors contain 44 (hpGRF-44), 40 (hpGRF-40), and 37 (hpGRF-37) amino acids in identical sequences from their NH2 termini. High pressure liquid chromatography of the native peptides and their synthetic replicates showed that hpGRF-37 and hpGRF-40 possess free carboxyl termini while that of hpGRF-44 is amidated. The structure of hpGRF-44 was established as: Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr-Arg-Lys-Val-Leu-Gly-Gln-Leu-Ser-Ala Arg-Lys-Leu-Leu-Gln-Asp-Ile-Met-Ser-Arg-Gln-Gln-Gly-Glu-Ser-Asn-Gln-Glu-Arg-Gly Ala-Arg-Ala-Arg-Leu-NH2.
通过使用气相序列分析仪对完整肽及其溴化氰消化片段进行序列分析，确定了三种具有高内在生长激素释放活性、源自一例导致肢端肥大症的人胰腺癌的多肽的一级结构。这三种人胰腺生长激素释放因子从其氨基末端起含有44个（hpGRF - 44）、40个（hpGRF - 40）和37个（hpGRF - 37）相同序列的氨基酸。天然肽及其合成复制品的高压液相色谱分析表明，hpGRF - 37和hpGRF - 40具有游离羧基末端，而hpGRF - 44的羧基末端是酰胺化的。hpGRF - 44的结构确定为：Tyr - Ala - Asp - Ala - Ile - Phe - Thr - Asn - Ser - Tyr - Arg - Lys - Val - Leu - Gly - Gln - Leu - Ser - Ala Arg - Lys - Leu - Leu - Gln - Asp - Ile - Met - Ser - Arg - Gln - Gln - Gly - Glu - Ser - Asn - Gln - Glu - Arg - Gly Ala - Arg - Ala - Arg - Leu - NH2。
