Gubler U, Monahan J J, Lomedico P T, Bhatt R S, Collier K J, Hoffman B J, Böhlen P, Esch F, Ling N, Zeytin F, Brazeau P, Poonian M S, Gage L P
Proc Natl Acad Sci U S A. 1983 Jul;80(14):4311-4. doi: 10.1073/pnas.80.14.4311.
Molecular cloning has established the primary structures of two precursors of the human pancreas growth hormone-releasing factor (hpGRF-44), somatocrinin. Both polypeptides contain the sequence of hpGRF-44 flanked by basic processing sites. Furthermore, the precursors include a putative signal sequence and a carboxyl-terminal amidation signal for hpGRF-44. The two forms of mRNA code for pre-pro-GRF-107 and pre-pro-GRF-108. Pre-pro-GRF-108 differs from pre-pro-GRF-107 by the insertion of a serine in the carboxyl-terminal portion of the precursor. In vitro translation of tumor poly(A)+ RNA followed by immunoprecipitation with GRF-specific antiserum and gel electrophoresis showed the molecular weight of preprosomatocrinin to be approximately 13,000, which is in good agreement with the molecular weight deduced from the sequences of the cDNA clones.
分子克隆确定了人类胰腺生长激素释放因子（hpGRF - 44）即生长激素释放素两种前体的一级结构。两种多肽均含有hpGRF - 44序列，两侧为碱性加工位点。此外，前体还包括一个假定的信号序列和hpGRF - 44的羧基末端酰胺化信号。两种形式的mRNA编码前 - 前体 - GRF - 107和前 - 前体 - GRF - 108。前 - 前体 - GRF - 108与前 - 前体 - GRF - 107的不同之处在于在前体的羧基末端部分插入了一个丝氨酸。肿瘤多聚腺苷酸加尾RNA的体外翻译，随后用GRF特异性抗血清进行免疫沉淀和凝胶电泳显示，前体生长激素释放素的分子量约为13,000，这与从cDNA克隆序列推导的分子量高度一致。
Zotero 插件
