Koppi T A, Halliday W J
Cell Immunol. 1983 Feb 15;76(1):29-38. doi: 10.1016/0008-8749(83)90345-3.
Spleen cells from mice bearing methylcholanthrene-induced tumors were cultured for 2 days without further stimulation. Blocking factors were consistently detected in culture supernatants by their ability to suppress leukocyte adherence inhibition reactions between soluble tumor antigens and peritoneal cells of tumor-bearing mice. The blocking factors were specific for individual tumors. The cellular origin of these factors was investigated by depleting the spleen cell population of various cell types before culturing. The cells involved were removed by treatment with antibodies to certain membrane markers (Thy-1, Ly-2, Ia, I-J) but not by anti-Ly-1 antibodies. Removal of adherent cells also prevented production of blocking factors, which was restored by reconstitution with syngeneic but not allogeneic cells from normal mice. The normal reconstituting cells were shown to bear Ia, but not I-J or IgM. This indicates that blocking factors (previously shown to have I-J determinants in their molecules) originate from suppressor T lymphocytes (Thy-1+, Ly-1-2+, I-J+), with macrophages (I-J-, Ia+) in the role of accessory cells.
将携带甲基胆蒽诱导肿瘤的小鼠的脾细胞在无进一步刺激的情况下培养2天。通过其抑制可溶性肿瘤抗原与荷瘤小鼠腹腔细胞之间白细胞黏附抑制反应的能力，在培养上清液中持续检测到阻断因子。这些阻断因子对单个肿瘤具有特异性。在培养前通过耗尽各种细胞类型的脾细胞群体来研究这些因子的细胞起源。通过用针对某些膜标记物（Thy-1、Ly-2、Ia、I-J）的抗体处理来去除相关细胞，但用抗Ly-1抗体则不能。去除贴壁细胞也会阻止阻断因子的产生，通过用正常小鼠的同基因而非异基因细胞重建可恢复阻断因子的产生。正常的重建细胞显示带有Ia，但不带有I-J或IgM。这表明阻断因子（先前已证明其分子中具有I-J决定簇）起源于抑制性T淋巴细胞（Thy-1+、Ly-1-2+、I-J+），巨噬细胞（I-J-、Ia+）起辅助细胞的作用。
